Characterization of full-length MHC class II sequences in Indonesian and Vietnamese cynomolgus macaques

Wisconsin National Primate Research Center, University of Wisconsin-Madison, USA.
Immunogenetics (Impact Factor: 2.23). 05/2011; 63(9):611-8. DOI: 10.1007/s00251-011-0537-5
Source: PubMed


In recent years, the use of cynomolgus macaques in biomedical research has increased greatly. However, with the exception of the Mauritian population, knowledge of the MHC class II genetics of the species remains limited. Here, using cDNA cloning and Sanger sequencing, we identified 127 full-length MHC class II alleles in a group of 12 Indonesian and 12 Vietnamese cynomolgus macaques. Forty two of these were completely novel to cynomolgus macaques while 61 extended the sequence of previously identified alleles from partial to full length. This more than doubles the number of full-length cynomolgus macaque MHC class II alleles available in GenBank, significantly expanding the allele library for the species and laying the groundwork for future evolutionary and functional studies.

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Available from: Ericka A Becker, May 16, 2014
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    • "Seven alleles were also detected in animals of Vietnamese descent. This confirms earlier findings of shared class II alleles in animals of Vietnamese and Indonesian descent (Creager et al. 2011). One allele was seen earlier in a Malaysian cohort (Aarnink et al. 2011). "
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