Serological Reactivity to Borrelia burgdorferi Sensu Lato in Dogs and Horses from Distinct Areas in Romania

Department of Infectious Diseases, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj, Romania.
Vector borne and zoonotic diseases (Larchmont, N.Y.) (Impact Factor: 2.3). 05/2011; 11(9):1259-62. DOI: 10.1089/vbz.2010.0254
Source: PubMed


Lyme disease is a perfect model of the complex relationship between host, vector, and the vector-borne bacteria. Both dogs and horses in Romania are exposed to infection. The aim of the present study was to assess the seroreactivity against Borrelia burgdorferi sensu lato in dogs and horses from different regions of Romania. 276 samples from dogs and 260 samples from horses located in different regions of Romania were analyzed by ELISA and IFA, respectively. The effect of several factors potentially affecting seroreactivity (location, age, gender, occupation, and vector exposition risk) was evaluated using Fisher's exact test (R 2.12.0). The overall prevalence of anti-Borrelia antibodies was 6.52% (18/276) in dogs, with a significantly higher positivity (46.15%, 6/13, p = 0.0005) recorded in a midcountry region. Seroreactivity was correlated with occupation, with working dogs being more exposed. The results may indicate that Lyme borreliosis foci are restricted to small areas, but further studies on Borrelia prevalence in tick populations are needed to confirm this hypothesis. In horses, a global seroprevalence of 11.92% (31/260) was observed. No correlations were found between positive results and age, sex, county, or occupation. This is the first serological survey on antibodies to B. burgdorferi sensu lato in Romanian dogs and horses.

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Available from: Andrei Daniel Mihalca, Mar 14, 2014
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    • "Moreover, B. burgdorferi s.l. was recently reported from Romania in the tissues of wild mustelid carnivores (Gherman et al., 2012). Indirect detection has also been recorded by seroprevalence studies in humans (Hristea et al., 2001; Persec˘ a et al., 2008) or domestic animals (Kiss et al., 2011; Mircean et al., 2012). Habitat variety of Romania (Donit¸˘ a et al., 2005) accounts for relatively high host diversity of I. ricinus, as compared to neighboring countries (Feider, 1965), many of these being reservoir hosts. "
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    ABSTRACT: The paper reports the prevalence and geographical distribution of Borrelia burgdorferi sensu lato (s.l.) and its genospecies in 12,221 questing Ixodes ricinus ticks collected at 183 locations from all the 41 counties of Romania. The unfed ticks were examined for the presence of B. burgdorferi s.l. by PCR targeting the intergenic spacer 5S–23S. Reverse line blot hybridization (RLB) and restriction fragment length polymorphism (RFLP) analysis were performed for identification of B. burgdorferi genospecies. The overall prevalence of infection was 1.4%, with an average local prevalence between 0.75% and 18.8%. B. burgdorferi s.l. was found in ticks of 55 of the 183 localities. The overall prevalence B. burgdorferi s.l. in ticks in the infected localities was 3.8%. The total infection prevalence was higher in female ticks than in other developmental stages. Three Borrelia genospecies were detected. The most widely distributed genospecies was B. afzelii, followed by B. garinii and B. burgdorferi sensu stricto (s.s.). The study is the first countrywide study and the first report of B. burgdorferi s.s. in Romania. The distribution maps show that higher prevalences were recorded in hilly areas, but Lyme borreliosis spirochetes were also present in forested lowlands, albeit with a lower prevalence.
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    • "In recent years, several authors have published the results of serological surveys on the presence of B. burgdorferi s.l. antibodies in horses from different European countries showing seroprevalence values ranging from 6% to up to 48% (Stefancíková et al., 2000; Egenvall et al., 2001; Hansen et al., 2010; Kiss et al., 2011). Given the scant information on the presence of B. burgdorferi infection s.l. in Italian horses nor on the genetic identity of the isolates, the present work aimed to investigate the occurrence of the infection in animals selected from an area of the Lazio region (Central Italy) with a high risk of I. ricinus. "
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    ABSTRACT: The aim of the study was to investigate Borrelia burgdorferi sensu lato (s.l.) infection in horses exposed to heavy tick infestations. Blood samples of 98 healthy horses from 5 stud farms were examined by SNAP(®) 4D× and PCR to detect antibodies against B. burgdorferi s.l. and Borrelia DNA, respectively. Ten samples (15.3%) were antibody positive and 5 samples (5.1%) were both antibody and PCR positive. Sequence analysis showed the highest homology with the B. lusitaniae genospecies. No differences were found between sexes and stud farms, while age was significantly related to seropositivity (p<0.05). Our data confirms the presence of B. lusitaniae infection in horses, previously not clearly demonstrated.
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    • "(Pejchalová et al. 2006; Kybicová et al. 2009), and the U.S. 5.1% (Bowman et al. 2009). In accordance with the literature, no relationship has been established between seroreactivity to B. burgdorferi s.l. and gender or age (Delgado and Cármenes 1995; S ˇ tefan cíková et al. 2008; Couto et al. 2010; Kiss et al. 2011). The lack of correlation may be a consequence of the limited persistence of anti-B. "
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