A fine balance between CCNL1 and TIMP1 contributes to the development of breast cancer cells

College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xi'an Road, Changchun 130062, PR China.
Biochemical and Biophysical Research Communications (Impact Factor: 2.3). 06/2011; 409(2):344-9. DOI: 10.1016/j.bbrc.2011.05.021
Source: PubMed


Cyclin L1 (CCNL1) and tissue inhibitor of matrix metalloproteinase-1 (TIMP1) are candidate genes involved in several types of cancer. However, the expression of CCNL1 and the relationship between CCNL1 and TIMP1 in breast cancer cells is unknown. Using patients' breast cancer tissues, the expression of CCNL1 and TIMP1 was measured by cDNA microarray and further confirmed by real-time RT-PCR and western blotting. Overexpression or repression of CCNL1 and TIMP1, individually or together, was performed in breast cancer MDA-MB-231 cells by transient transformation methods to investigate their role in breast cancer cell growth. Simultaneously, mRNA and protein expression levels of CCNL1 and TIMP1 were also measured. CCNL1 and TIMP1 expression was significantly elevated in breast cancer tissues compared with that in peri-breast cancer tissues of patients by cDNA microarray and these results were further confirmed by real-time RT-PCR and western blotting. Interestingly, in vitro experiments showed a stimulatory effect of TIMP1 and an inhibitory effect of CCNL1 on growth of MDA-MB-231 cells. Co-expression or co-repression of these two genes did not affect cell growth. Overexpression of CCNL1 and TIMP1 individually induced overexpression of each other. These data demonstrate that there is a fine balance between CCNL1 and TIMP1, which may contribute to breast cancer development.

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    • "A cDNA microarray chip (22K Human Genome Array Chip; CapitalBio, Co., Ltd., Beijing, China) was used for the tumor or peritumor tissue samples in this study. The cDNA microarray chip was constructed by CapitalBio Co., Ltd. as previously described by our laboratory (26). Briefly, the total RNA was extracted using TRIzol reagent and further purified using a NucleoSpin® RNA clean-up kit (Takara, Otsu, Shiga, Japan). "
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