Article

Anti-inflammatory activity of edible oyster mushroom is mediated through the inhibition of NF-κB and AP-1 signaling

Cancer Research Laboratory, Methodist Research Institute, Indiana University Health, Indianapolis, 46202, USA.
Nutrition Journal (Impact Factor: 2.6). 05/2011; 10(1, article 52):52. DOI: 10.1186/1475-2891-10-52
Source: PubMed

ABSTRACT

Mushrooms are well recognized for their culinary properties as well as for their potency to enhance immune response. In the present study, we evaluated anti-inflammatory properties of an edible oyster mushroom (Pleurotus ostreatus) in vitro and in vivo.
RAW264.7 murine macrophage cell line and murine splenocytes were incubated with the oyster mushroom concentrate (OMC, 0-100 μg/ml) in the absence or presence of lipopolysacharide (LPS) or concanavalin A (ConA), respectively. Cell proliferation was determined by MTT assay. Expression of cytokines and proteins was measured by ELISA assay and Western blot analysis, respectively. DNA-binding activity was assayed by the gel-shift analysis. Inflammation in mice was induced by intraperitoneal injection of LPS.
OMC suppressed LPS-induced secretion of tumor necrosis factor-α (TNF-α, interleukin-6 (IL-6), and IL-12p40 from RAW264.7 macrophages. OMC inhibited LPS-induced production of prostaglandin E2 (PGE2) and nitric oxide (NO) through the down-regulation of expression of COX-2 and iNOS, respectively. OMC also inhibited LPS-dependent DNA-binding activity of AP-1 and NF-κB in RAW264.7 cells. Oral administration of OMC markedly suppressed secretion of TNF-α and IL-6 in mice challenged with LPS in vivo. Anti-inflammatory activity of OMC was confirmed by the inhibition of proliferation and secretion of interferon-γ (IFN-γ), IL-2, and IL-6 from concanavalin A (ConA)-stimulated mouse splenocytes.
Our study suggests that oyster mushroom possesses anti-inflammatory activities and could be considered a dietary agent against inflammation. The health benefits of the oyster mushroom warrant further clinical studies.

Download full-text

Full-text

Available from: Daniel Sliva
  • Source
    • "For " mould " fungi as defined above, glucan is predominantly present in a triple-helical conformation (Williams 1997; Williams et al. 2005). Some Basidiomycetes produce both β-1,3-D-glucan and β-1,6-D- glucan that have anti-inflammatory properties in vitro and in vivo (Jedinak et al. 2011; Pacheco-Sanchez et al. 2006). Fungi from different taxonomic groups produce varying relative amounts of β-1,3-D-glucan compared to other glucans (Odabasi et al. 2006). "
    [Show abstract] [Hide abstract]
    ABSTRACT: From the epidemiological studies conducted on the effect of mould and dampness on health a decade ago, the role of toxin-producing fungi in damp and mouldy buildings involved opinion more than evidence. Very little was known about the metabolites that were produced by the fungi that grew on damp building materials, and almost nothing had been reported on their occurrence in buildings. As a consequence, the focus was on speculations involving the fungal toxins that occur in agriculture. Over the past decade, particularly in the last 5 years, considerable progress has been made concerning the relevant toxins from fungi that grow on damp building materials. This paper summarizes the available data on the low-molecular-weight toxins reliably known from fungi common on damp building materials, the toxins that have been measured on mouldy building materials and the new understanding of the role that they play in the documented health effects of individuals living and working in damp and mouldy buildings.
    Full-text · Article · Nov 2014 · Applied Microbiology and Biotechnology
  • Source
    • "Carrageenan-induced paw edema is a well-known model of acute inflammation that consists of a biphasic inflammatory response and a number of mediators participate in this inflammatory response.[1316] Following the injection of carrageenan into the rat paw, several mediators are sequentially released, including histamine, serotonin, and bradykinin in the initial phase (0-1 h), followed by an increase in the production of prostaglandins (PGs) through the activation of cyclooxygenase-2 (COX-2) and the release of nitric oxide (NO) in the second phase (1-6 h).[171819] It is well-known that reactive oxygen species, NO and PGE2 are considered as inflammatory factors and play important roles in the tissue damage by inflammation.[2021] "
    [Show abstract] [Hide abstract]
    ABSTRACT: Background: Derivatives of pyridine-4-one act as iron chelators and possess various pharmacological effects such as antifungal, antimalarial, antiviral, anti-inflammatory, and analgesic effects. The aim of our study was to evaluate the anti-inflammatory effects of the three new derivatives of pyridine-4-one. Materials and Methods: Carrageenan-induced paw edema in rats and croton oil-induced ear edema in mice were used to evaluate the anti-inflammatry effects of three 3-hydroxy-pyridine-4-one derivatives (compounds A, B, and C). Compound A (10, 20 mg/kg), compound B (200, 400 mg/kg), and compound C (100, 200 mg/kg), vehicle (1 mL/kg), and indomethacin as standard drug (10 mg/kg) were injected intraperitoneally 30 min prior to carrageenan injection and 4 h later, the paw volume was measured using a mercury plethysmograph. The maximum dose of each test compound was used in the croton oil-induced ear edema test. Results: All compounds showed significant anti-inflammatory activity in both tests. On a molar basis, compound A had the greatest potency, which may be due to the presence of a benzyl group substitution on the pyridine ring. Conclusions: Because cyclooxygenase and lipoxygenase as key enzymes of the inflammation pathway are heme-dependent, it seems that the anti-inflammatory effect of derivatives of pyridine-4-one may be related to their iron chelating properties. However, more investigations are needed to find out their exact mechanism of actions.
    Full-text · Article · May 2014
  • Source
    • "Wang[26]; Gao[86]; Jedinak[87]Pleurotus tuber-regium 32-kDa N-acetylglucosamine-binding Exhibits hemagglutinating activity toward trypsinized rabbit erythrocytes but not toward untrypsinized rabbit erythrocytes. Wang[88]Polyporus adusta 12-kDa subunits, Homodimeric Turanose is the most potent inhibitory sugar Antiproliferative activity toward tumor cell lines and mitogenic activity toward splenocytes Wang[89]Polyporus squamosus 28-kDa subunits, Homodimeric NeuNAcα2,6βgalactosyl residues Can be a valuable tool for glycobiological studies in biomedical and cancer research Mo[90]Psathyrella velutina 40-kDa, Monomeric having a regular seven-bladed β -propeller fold N-acetylglucosamine and N-acetylneuraminic acid specific Used in detection of glycosylation abnormality in rheumatoid IgG Cioci[91]; Kochibe[92]Russula delica 60-kDa, Homodimeric Inulin and O-nitrophenyl-beta-D-galactopyranoside "
    [Show abstract] [Hide abstract]
    ABSTRACT: Mushrooms are famous for their nutritional and medicinal values and also for the diversity of bioactive compounds they contain including lectins. The present review is an attempt to summarize and discuss data available on molecular weights, structures, biological properties, N-terminal sequences and possible applications of lectins from edible mushrooms. It further aims to update and discuss/examine the recent advancements in the study of these lectins regarding their structures, functions, and exploitable properties. A detailed tabling of all the available data for N-terminal sequences of these lectins is also presented here.
    Full-text · Article · Jan 2014 · Molecules
Show more