Zhou, Y. et al. Description of Sinomonas soli sp. nov., reclassification of Arthrobacter echigonensis and Arthrobacter albidus (Ding et al. 2009) as Sinomonas echigonensis comb. nov. and Sinomonas albida comb. nov., respectively, and emended description of the genus Sinomonas. Int. J. Syst. Evol. Microbiol. 62, 764-769

Quality and Standards Institute of Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang 310021, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 05/2011; 62(Pt 4):764-9. DOI: 10.1099/ijs.0.030361-0
Source: PubMed


A novel actinomycete strain, designated CW 59T, was isolated from a polluted forest soil sample in Anhui Province, China. Cells were strictly aerobic, non-motile, bent rods. The strain grew optimally at 30-37 °C and pH 6.0-8.0. The major fatty acids were ai-C15:0 (34.7 %), i-C15:0 (11.6 %) and ai-C17:0 (14.9 %); the predominant respiratory quinone was MK-9(H2), with MK-8(H2) present as a minor component. The polar lipid composition of strain CW 59T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two glycolipids and phosphatidylmonomethylethanolamine (minor component). The whole-cell sugars contained galactose, mannose, ribose and glucose; the major amino acids of the cell-wall were lysine, alanine and glutamic acid. The genomic DNA G+C content was 66.9 mol%. Phylogenetic analysis showed that CW 59T belonged to the genus Sinomonas and grouped with members of the species Sinomonas atrocyanea, Sinomonas flava, Arthrobacter echigonensis and Arthrobacter albidus. 16S rRNA gene sequence similarities of CW 59T to S. atrocyanea DSM 20127T, S. flava CW 108T, A. echigonensis LC10T and A. albidus LC13T were 99.5, 99.3, 98.2 and 98.0 %, respectively. DNA-DNA hybridization of the isolate showed relatedness values of 58.3 % (DSM 20127T), 41.8 % (CW 108T), 21.6 % (LC10T) and 25.5 % (LC13T) with its four closest neighbours. The taxonomic relationships of strains LC10T and LC13T with the genus Sinomonas were further clarified by means of a direct experimental comparison; results showed that strains LC10T and LC13T showed the same major fatty acid, polar lipid, cell-wall amino acid, whole-cell sugar and respiratory quinone compositions as members of the genus Sinomonas. Based on phenotypic, chemotaxonomic and phylogenetic analysis, it is proposed that: strain CW 59T represents a novel species of the genus Sinomonas, Sinomonas soli sp. nov., with CW 59T (=CCTCC AB 207193T=KCTC 19389T) as the type strain; and the type strains of A. echigonensis and A. albidus should be reclassified as Sinomonas echigonensis comb. nov. and Sinomonas albida comb. nov., respectively. An emended description of the genus Sinomonas is given.

  • Source
    • "sequence similarity to other Sinomonas species. Most of the members of Sinomonas genus were isolated from habitats including polluted forest soil and filtration substrate (Zhou et al., 2012; Bao et al., 2015). Furthermore, previous study has also discovered oil-degrading potential carried by Sinomonas species isolated from Indian Ocean sea surface (Wu et al., 2010). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The amylolytic actinobacterium, Sinomonas humi MUSC 117(T) was isolated from intertidal soil from Kuantan, Malaysia. MUSC 117(T) exhibited significant starch hydrolysis activity and was chosen for further analysis. Here we report approximately 4.4Mbp high quality genome sequence of MUSC 117(T). Availability of the genome sequence will contribute to better understanding for the strain and allow further exploitation of its biotechnological potential. Copyright © 2015. Published by Elsevier B.V.
    Full-text · Article · May 2015 · Marine Genomics
  • Source
    • "At the time of writing, the genus Sinomonas comprised seven species ( the type species S. flava and S. atrocyanea (Zhou et al., 2009), S. albida, S. echigonensis, S. soli, 'Sinomonas notoginsengisoli' and 'Sinomonas mesophila' (Zhou et al., 2012; Prabhu et al., 2014; Zhang et al., 2014). Members of these species were isolated from habitats such as polluted forest soil and filtration substrate (Ding et al., 2009; Zhou et al., 2009, 2012). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Strain MUSC 117T was isolated from mangrove soil of the Tanjung Lumpur forest in Pahang, Malaysia. This bacterium was yellowish white pigmented, Gram-staining-positive, rod-coccus shaped and non-motile. Based on the 16S rRNA gene sequence, strain MUSC 117T exhibited highest sequence similarity to Sinomonas atrocyanea DSM 20127T (98.0 %), S. albida LC 13T (97.9 %), S. soli CW 59T (97.8 %) and lower (<97.6 %) sequence similarity to other Sinomonas species. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 27 %) between strain MUSC 117T to its closely related species. Chemotaxonomically, the peptidoglycan type was A3α, containing the amino acids lysine, serine, glycine, alanine, glutamic acid and muramic acid. The whole-cell sugars detected were rhamnose, ribose, glucose, galactose and lower amount of mannose. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and five unidentified glycolipid. The major fatty acids (>10.0 %) of the cell membrane were anteiso-C15 : 0 (39.4 %), C18 : 1 ω7c (17.7 %) , anteiso-C17 : 0 (17.2 %) and iso-C16:0 (11.4 %). The predominant respiratory quinones detected were MK-9(H2) and MK-9. DNA G + C ratio was 67.3 mol %. The comparison of BOX-PCR fingerprint indicated that strain MUSC 117T represented a unique DNA profile. Based on polyphasic approach and results showed that strain MUSC 117T represent a novel species of the genus Sinomonas, for which the name Sinomonas humi sp. nov. is proposed. The type strain of Sinomonas humi is MUSC 117T (= DSM 29362T = MCCC 1K00410T = NBRC 110653T).
    Full-text · Article · Jan 2015 · International Journal of Systematic and Evolutionary Microbiology
  • Source
    • "In this study, it is evident that some genera not commonly found in the mangrove environment were discovered, such as Streptacidiphilus, Sinomonas, Terrabacter, and Leifsonia. So far, the different species of Streptacidiphilus were isolated from area such as Pinus soils [44], acidic rhizosphere soil [45], and rice field soil [46], while Sinomonas was mostly discovered in forest soils [47], polluted forest soil [48], and volcanic soil [49]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The aim of this study was to isolate and identify Actinobacteria from Malaysia mangrove forest and screen them for production of antimicrobial secondary metabolites. Eighty-seven isolates were isolated from soil samples collected at 4 different sites. This is the first report to describe the isolation of Streptomyces, Mycobacterium, Leifsonia, Microbacterium, Sinomonas, Nocardia, Terrabacter, Streptacidiphilus, Micromonospora, Gordonia, and Nocardioides from mangrove in east coast of Malaysia. Of 87 isolates, at least 5 isolates are considered as putative novel taxa. Nine Streptomyces sp. isolates were producing potent antimicrobial secondary metabolites, indicating that Streptomyces isolates are providing high quality metabolites for drug discovery purposes. The discovery of a novel species, Streptomyces pluripotens sp. nov. MUSC 135(T) that produced potent secondary metabolites inhibiting the growth of MRSA, had provided promising metabolites for drug discovery research. The biosynthetic potential of 87 isolates was investigated by the detection of polyketide synthetase (PKS) and nonribosomal polyketide synthetase (NRPS) genes, the hallmarks of secondary metabolites production. Results showed that many isolates were positive for PKS-I (19.5%), PKS-II (42.5%), and NRPS (5.7%) genes, indicating that mangrove Actinobacteria have significant biosynthetic potential. Our results highlighted that mangrove environment represented a rich reservoir for isolation of Actinobacteria, which are potential sources for discovery of antimicrobial secondary metabolites.
    Full-text · Article · Aug 2014
Show more