Approaches for cytogenetic and molecular analyses of small flow-sorted cell populations from childhood leukemia bone marrow samples

Department of Clinical Immunology, section 7631, University Hospital Rigshospitalet, Copenhagen, Denmark.
Journal of immunological methods (Impact Factor: 1.82). 06/2011; 369(1-2):69-73. DOI: 10.1016/j.jim.2011.04.007
Source: PubMed


Discordances between minimal residual disease estimates obtained by different methods are a problem in childhood acute lymphoblastic leukemia. We aimed to optimize methods allowing the biological exploration of such discrepancies, i.e. the combination of flow-sorting of small immunophenotypically defined cell populations with subsequent analyses of leukemia-associated cytogenetic and molecular marker. The approaches described here optimize the use of the same tube of unfixed, antibody-stained BM cells for flow-sorting of small cell populations and subsequent exploratory FISH and PCR-based analyses.

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