CCR2-64I gene polymorphism increase susceptibility to oral cancer

Department of Otorhinolaryngology-Head and Neck Surgery, Changhua Christian Hospital, Changhua, Taiwan.
Oral Oncology (Impact Factor: 3.61). 05/2011; 47(7):577-82. DOI: 10.1016/j.oraloncology.2011.04.008
Source: PubMed


The purpose of this study was to investigate the impact of MCP-1 and its receptor CCR2 gene polymorphisms on the susceptibility and clinicopathological characteristics of oral cancer, as well as the synergistic effect between these gene polymorphisms and well-known risk factors including alcohol, tobacco, and areca consumptions. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique for polymorphism analysis, 344 healthy controls and 216 oral cancer patients were recruited to reveal a significant association between V64I CCR2 gene polymorphism and oral cancer susceptibility. After adjusting for other confounders, individuals with GA (AOR=1.84; 95%CI=1.10-3.20) or at least one A allele (AOR=1.78; 95%CI=1.05-3.02) had a higher risk for oral cancer, compared to GG genotypes. Moreover, results also revealed that for subjects with GA or at least one A allele of V64I CCR2 gene polymorphism, those exposed to environmental risk factors possessed a significantly higher risk for oral cancer than those unexposed subjects. Therefore, genetic polymorphism of CCR2-64I may contribute to the susceptibility to oral cancer.

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    • "After HWE test, 3 articles (Saenz-Lopez et al., 2008; Attar et al., 2010; Gu et al., 2011) were deviated from HWE. Thus, a total of 11 case– control studies were extracted (Vazquez- Lavista et al., 2009; Narter et al., 2010; Yang et al., 2010; Yeh et al., 2010; Chen et al., 2011; Kruszyna et al., 2011; Kucukgergin et al., 2012a; Kucukgergin et al., 2012b; Bektas-Kayhan et al., 2012; Singh et al., 2012; Wu et al., 2013). The characteristics of each case–control study are listed in Table 1. "
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    ABSTRACT: Background: The 2518 A/G polymorphism in the MCP-1 gene has been extensively studied for associations with cancer; however, results from replication studies have been inconsistent. The aim of this investigation was to determine links with risk of cancer by meta-analysis. Methods: We searched Pubmed, Embase, CNKI, Weipu and Wanfang databases, covering all case-control studies until March, 2013. Statistical analyses were performed using the Revman 5.0 software. Results: A total of 11 case-control studies met our inclusion criteria, including 1,422 cases and 2,237 controls. The results indicated that the MCP-1 2518 gene polymorphism had no association with cancer risk overall (GG vs.GA+ AA: OR = 0.89, 95%CI = 0.61-1.28, P = 0.52). However, in the subgroup analysis by ethnicity, a decrease of cancer risk was found in Asian populations (GG vs.GA+ AA: OR = 0.79, 95%CI = 0.63-0.99, P = 0.04). Conclusion: This meta-analysis suggested that the 2518A/G polymorphism of MCP-1 gene is associated with risk of cancer among Asian, but not in Caucasian populations.
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    ABSTRACT: We aimed to investigate a possible association of the MCP-1 and CCR2 polymorphisms with the risk of developing oral squamous cell carcinoma (OSCC). MCP-1 A2518G and CCR2 V64I gene polymorphisms were performed by polymerase chain reaction and restriction fragment length polymorphism, in 129 patients with OSCC and 140 healthy control subjects. Individuals who had G allele and GG genotype of MCP-1, and 64I allele and wt/64I genotype of CCR2 had increased risk for OSCC (P<0.05.) In contrast, individuals with CCR2 wt/wt genotype seem to be protected from OSCC (P < 0.01). Haplotype analysis revealed that MCP-1G: CCR2 64I haplotype frequencies were significantly higher in patients than those of controls (P = 0.001). We can suggest that the G allele of MCP-1 and 64I allele of CCR2 may be risk factors for OSCC.
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