Rapid profiling and identification of triterpenoid saponins in crude extracts from Albizia julibrissin Durazz by ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time of flight tandem mass spectrometry. J Pharm Biomed Anal

Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, No. 88 Yuquan Road, Tianjin 300193, PR China. hanlifeng
Journal of pharmaceutical and biomedical analysis (Impact Factor: 2.98). 07/2011; 55(5):996-1009. DOI: 10.1016/j.jpba.2011.04.002
Source: PubMed


Ultra high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC/ESI-Q-TOF-MS/MS) was applied to separate and identify triterpenoid saponins in crude extract from the stem bark of Albizia julibrissin Durazz. The molecular weights were determined by comparing quasi-molecular ions [M+NH(4)](+) in positive mode and [M-H](-) and [M-2H](2-) ions in negative mode. The MS/MS spectra of the [M-H](-) ions for saponins provided a wealth of structural information related to aglycone skeletons, sugar types and linked sequence. On the basis of the fragmentation behavior of known saponins isolated before, saponins from this plant were identified, even though references were not available. As a result, a total of twenty-eight saponins in the crude extract were identified, which all had a common basic skeleton of the triterpene oleanolic acid and eight of them were new compounds.

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    • "Up to data, UPLC analysis has the advantage of the high chromatographic peak capacity, fast analysis, and good sensitivity as well as resolution in comparison with other isolation methods (Guan, Lai, & Li, 2007). In addition, the UPLC coupled with mass spectrometry performs adequate structural information of multiple compounds (Han et al., 2011). Based on the above considerations, our work was designed to get available information regarding saponins in legume seeds using UPLC–PDA–ESI/MS analysis. "
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