Selective Biomolecular Nanoarrays for Parallel Single-Molecule Investigations

Department of Applied Physics & Applied Mathematics, Columbia University, New York, New York 10027, USA.
Journal of the American Chemical Society (Impact Factor: 12.11). 05/2011; 133(20):7656-9. DOI: 10.1021/ja201031g
Source: PubMed


The ability to direct the self-assembly of biomolecules on surfaces with true nanoscale control is key for the creation of functional substrates. Herein we report the fabrication of nanoscale biomolecular arrays via selective self-assembly on nanopatterned surfaces and minimized nonspecific adsorption. We demonstrate that the platform developed allows for the simultaneous screening of specific protein-DNA binding events at the single-molecule level. The strategy presented here is generally applicable and enables high-throughput monitoring of biological activity in real time and with single-molecule resolution.

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