Massilia flava sp nov., isolated from soil

School of Life Science, Beijing Institute of Technology, Beijing, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 04/2011; 62(Pt 3):580-5. DOI: 10.1099/ijs.0.031344-0
Source: PubMed


A Gram-stain-negative, rod-shaped bacterium, designated strain Y9(T), was isolated from a soil sample collected in Ningxia Province in China and was characterized to determine its taxonomic position. Strain Y9(T) contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (C(16:1)ω7c and/or iso-C(15:0) 2-OH) and C(16:0). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain Y9(T) was 68.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Massilia. Levels of 16S rRNA gene sequence similarity between strain Y9(T) and the type strains of recognized Massilia species ranged from 95.2 to 98.2%, the highest values being with Massilia albidiflava 45(T) (98.2%) and Massilia lutea 101(T) (98.0%). However, levels of DNA-DNA relatedness between strain Y9(T) and M. albidiflava KCTC 12343(T) and M. lutea KCTC 12345(T) were 37 and 26%, respectively. Strain Y9(T) was clearly differentiated from its nearest phylogenetic relatives in the genus Massilia based on phenotypic, chemotaxonomic and phylogenetic properties. Therefore, strain Y9(T) is considered to represent a novel species of the genus Massilia, for which the name Massilia flava sp. nov. is proposed. The type strain is Y9(T) (=CGMCC 1.10685(T) =KCTC 23585(T)).

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    • "The genus Massilia was first proposed by La Scola et al. (1998), who described an isolate from the blood of an immunocompromised patient with cerebella lesions. Subsequently, many members of this genus have been isolated from various environments, including soil (Zhang et al., 2006; Zul et al., 2008; Wang et al., 2012). Taxonomically, the genus belongs to the family Oxalobacteraceae, class Betaproteobacteria. "
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    ABSTRACT: A Gram-negative, short, rod-shaped bacterium, TSA1(T), was isolated from forest soil collected at Kyonggi University, South Korea. Assessment of 16S rRNA gene sequence similarity indicated that the strain is related to Massilia niastensis 5516S-1(T) (98.3%), M. haematophila CCUG 38318(T) (97.9%), M. aerilata 5516S-11(T) (97.9%), M. tieshanensis TS3(T) (97.6%), and M. varians CCUG 3529(T) (97.1%). Colonies grown on Reasoner's 2A agar at 30°C for 2 days were transparent, white, round, smooth, and glossy. The cells grew at 10-42°C (optimum: 25-37°C) and pH 5-9 (optimum: 5-9) and in 0-2% NaCl (optimum: 0-1%). TSA1(T) was able to grow on trypticase soy and nutrient agar, but not on Luria-Bertani or MacConkey agar. The strain was catalase- and oxidasepositive and able to degrade starch and casein, but not carboxymethyl cellulose. The predominant quinone of TSA1(T) was Q-8, the major fatty acids were summed feature 3 and C16:0, and the DNA G+C content was 66.7 mol%. Given these findings, we propose that this strain is a novel species of the genus Massilia. We suggest the name Massilia kyonggiensis sp. nov. (type strain, KACC 17471(T) =KEMB 9005-031(T) =JCM 19189(T)).
    Full-text · Article · May 2014 · The Journal of Microbiology
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    • "Therefore, Kämpfer et al. (2011) transferred all Naxibacter species to the genus Massilia. At the time of writing, a total of 18 Massilia species with validly published names have been described (La Scola et al., 1998; Gallego et al., 2006; Zhang et al., 2006; Zul et al., 2008; Weon et al., 2008, 2009, 2010; Kämpfer et al., 2011, 2012a; Wang et al., 2012). "
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    ABSTRACT: A Gram-negative, rod-shaped, aerobic, motile, with polar-flagella bacterium Y1243-1T was isolated from an ice core drilled from Yuzhufeng Glacier, Tibetan Plateau, China. 16S rRNA gene sequence of the novel isolate shares 94.7 % - 97.6 % homology with the type strains of the genus Massilia. The novel isolate is classified into the genus Massilia. The major fatty acids of strain Y1243-1T are summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) (43.98 %), C16 : 0 (27.86 %), C10 : 0 3-OH (7.10 %), C18 : 0 (6.95 %) and C18 : 1ω7c (5.01 %). The predominant isoprenoid quinone is Q-8. The DNA G+C content is 65.7 mol% (Tm). The major polar lipids of this bacterium are: phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. A number of phenotypic characteristics distinguished the novel isolate and type strains in the genus Massilia. Furthermore, in DNA-DNA hybridiazation tests, the bacterium Y1243-1T showed 45% relatedness with the closest type strain Massilia consociata CCUG 58010T. From the genotypic and phenotypic data, it is evident that the strain Y1243-1T represents a novel species of the genus Massilia, for which the name Massilia yuzhufengensis sp. nov. is proposed. The type strain is Y1243-1T (=KACC 16569T =CGMCC 1.12041T).
    Full-text · Article · Jul 2012 · International Journal of Systematic and Evolutionary Microbiology
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    • "mol%). The major polar lipids have been determined for some species of the genus as phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol (Kämpfer et al., 2011, 2012, 2008; Wang et al., 2012; Weon et al., 2010). "
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    ABSTRACT: A bacterial isolate, designated strain TS3(T), was isolated from soil collected from a metal mine in Tieshan District, Daye City, Hubei Province, in central China. Cells of this strain were Gram-negative, motile and rod-shaped. The strain had ubiquinone Q-8 as the predominant respiratory quinone, phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol as the major polar lipids and summed feature 3 (C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH), C(16 : 0) and C(18 : 1)ω7c as the major fatty acids. The G+C content was 65.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TS3(T) was most closely related to Massilia niastensis 5516S-1(T) (98.5 %), Massilia consociata CCUG 58010(T) (97.6 %), Massilia aerilata 5516S-11(T) (97.4 %) and Massilia varians CCUG 35299(T) (97.2 %). DNA-DNA hybridization revealed low relatedness between strain TS3(T) and M. niastensis KACC 12599(T) (36.5 %), M. consociata CCUG 58010(T) (27.1 %), M. aerilata KACC 12505(T) (22.7 %) and M. varians CCUG 35299(T) (46.5 %). On the basis of phenotypic and phylogenetic characteristics, strain TS3(T) belongs to the genus Massilia, but is clearly differentiated from other members of the genus. The strain represents a novel species, for which the name Massilia tieshanensis sp. nov. is proposed. The type strain is TS3(T) ( = CCTCC AB 2010202(T)  = KACC 14940(T)).
    Preview · Article · Nov 2011 · International Journal of Systematic and Evolutionary Microbiology
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