Fasciola hepatica and Schistosoma mansoni: Identification of Common Proteins by Comparative Proteomic Analysis

Biomedical Proteomics Facility, Department of Microbiology and Immunology, School of Medicine, Universidad Central del Caribe, Bayamón, Puerto Rico.
Journal of Parasitology (Impact Factor: 1.23). 03/2011; 97(5):852-61. DOI: 10.1645/GE-2495.1
Source: PubMed


It is not unusual to find common molecules among parasites of different species, genera, or phyla. When those molecules are antigenic, they may be used for developing drugs or vaccines that simultaneously target different species or genera of parasite. In the present study, we used a proteomic-based approach to identify proteins that are common to adult Fasciola hepatica and Schistosoma mansoni. Whole-worm extracts from each parasite were separated by 2-dimensional electrophoresis (2-DE), and digital images of both proteomes were superimposed using imaging software to identify proteins with identical isoelectric points and molecular weights. Protein identities were determined by mass spectrometry. Imaging and immunoblot analyses identified 28 immunoreactive proteins that are common to both parasites. Among these molecules are antioxidant proteins (thioredoxin and glutathione-S-transferase), glycolytic enzymes (glyceraldehyde 6-phosphate dehydrogenase and enolase), proteolytic enzymes (cathepsin-L and -D), inhibitors (Kunitz-type, Stefin-1), proteins with chaperone activity (heat shock protein 70 and fatty acid-binding protein), and structural proteins (calcium-binding protein, actin, and myosin). Some of the identified proteins could be used to develop drugs and vaccines against fascioliasis and schistosomiasis.

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Available from: Ana M Espino, Jul 02, 2014
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    • "It is now our interest to identify the individual molecules within FhES that induce the expansion of CD4+CD25+Foxp3 Tregs via the activation of M2-TGFβ secreting macrophages. Proteomics analysis revealed that FhES is a much less complex mix than SEA [47], [48]. Interestingly, the two major immune-modulatory molecules found in SEA, IPSE/α-1 [49] and glycoprotein ω-1 [50], are not found in FhES (although the carbohydrate moieties in FhES have yet to be characterised). "
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    ABSTRACT: Infections with helminth parasites prevent/attenuate auto-inflammatory disease. Here we show that molecules secreted by a helminth parasite could prevent Type 1 Diabetes (T1D) in nonobese diabetic (NOD) mice. When delivered at 4 weeks of age (coincident with the initiation of autoimmunity), the excretory/secretory products of Fasciola hepatica (FhES) prevented the onset of T1D, with 84% of mice remaining normoglycaemic and insulitis-free at 30 weeks of age. Disease protection was associated with suppression of IFN-γ secretion from autoreactive T cells and a switch to the production of a regulatory isotype (from IgG2a to IgG1) of autoantibody. Following FhES injection, peritoneal macrophages converted to a regulatory M2 phenotype, characterised by increased expression levels of Ym1, Arg-1, TGFβ and PD-L1. Expression of these M2 genetic markers increased in the pancreatic lymph nodes and the pancreas of FhES-treated mice. In vitro, FhES-stimulated M2 macrophages induced the differentiation of Tregs from splenocytes isolated from naïve NOD mice. Collectively, our data shows that FhES contains immune-modulatory molecules that mediate protection from autoimmune diabetes via the induction and maintenance of a regulatory immune environment.
    Full-text · Article · Jan 2014 · PLoS ONE
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    Full-text · Article · Jun 2012 · International Journal of Infectious Diseases
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    ABSTRACT: The trematodes Fasciola hepatica and F.gigantica can infect a broad range of hosts, and cause the disease Fasciolosis. Fasciolosis is a disease of economic importance, with livestock infection having a considerable impact on the agriculture industry. Human infection is now considered of public health importance and is hyperendemic in some regions. The incidence of animal and human infections is increasing in parts of the world. This review will provide an overview of our current understanding of Fasciola, from distribution, diversity and disease development, to current control measures and progress towards an effective vaccine. Fasciola excrete and secrete a broad range of molecules while residing in the host. The repertoire of molecules changes as the parasite matures, reflecting the different needs of the fluke during parasite development. These molecules, which are involved in important parasite functions, are often found in the parasites excretory-secretory material, and function at the host-parasite interface. Common among these molecules are antioxidents as well as a variety of proteases, including cathepsin proteases. The roles of these key molecules during Fasciola infection, including their ability to modulate host immune responses will be a focus of the review. The findings from recent transcriptome and proteome analysis will also be a discussed, as will the potential for RNA interference in characterisation of this parasite.
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