Effects of lycopene and apigenin on human umbilical vein endothelial cells in vitro under angiogenic stimulation

Health Sciences Research Centre, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Acta histochemica (Impact Factor: 1.71). 04/2011; 114(2):94-100. DOI: 10.1016/j.acthis.2011.03.004
Source: PubMed


Angiogenesis is the formation process of new blood vessels from preexisting vessels. Solid tumors need angiogenesis for growth and metastasis. The suppression of tumor growth by inhibition of neoangiogenic processes represents a potential approach to cancer treatment. Lycopene has powerful antioxidant capacities and anticarcinogenic properties. The aim of this study was to investigate the effects of lycopene on angiogenesis in vitro. For this reason, we measured in vitro angiogenesis in human umbilical vein endothelial cells including parameters of cell proliferation, tube formation, cell migration. Lycopene and apigenin were observed to block the endothelial cell proliferation in a dose-dependent manner. In addition, they significantly decreased the capillary-like tube lengths, tube formation and endothelial cell migration. This study provides indications that apigenin and lycopene, which are considered as chemopreventive agents, to be effective in vitro on endothelial cells and angiogenesis.

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Available from: Saadet Gümüşlü, Apr 21, 2015
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    • "Recent studies have shown that lycopene inhibits human umbilical vascular endothelial cell (HUVEC) migration and tube formation [53]. Moreover, high doses of lycopene can inhibit tumour growth in nude mice xenotransplanted with the PC-3 prostate carcinoma and Sk-Hep-1 hepatocellular carcinoma cell lines. "
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    • "In contrast, it was shown that prolonged tomato juice consumption (containing 47.1 mg/d lycopene) increases plasma lycopene concentrations without significantly affecting cell-mediated immunity in well-nourished elderly subjects. Lycopene has been shown to significantly decrease endothelial cell proliferation, migration and tube formation; the latter result suggests that lycopene may inhibit angiogenesis [34]. We previously showed that oral supplementation with lycopene decreases the number of lung tumors and the tumor cross-sectional area in athymic nude mice after tail vein injection with SK-Hep-1 cells, and this effect involves angiogenic factors, including up-regulation of IL-12 secretion [35]. "
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    • "Recently, lycopene has been shown to inhibit tube formation and migration in human umbilical vein endothelial cells (HUVECs) [27]. In addition, our previous studies have demonstrated that lycopene decreases the vascular endothelial growth factor (VEGF) plasma levels in nude mice transplanted with prostate tumor PC-3 cells and in nude mice injected via tail vein with hepatocarcinoma SK-Hep-1 cells [20, 28]. "
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