Amplified Fragment Length Polymorphism Reveals Specific Epigenetic Distinctions between Mycobacterium avium Subspecies paratuberculosis Isolates of Various Isolation Types

Department of Veterinary Pathobiology, Texas A&M University, Texas 77843-1114, USA.
Journal of clinical microbiology (Impact Factor: 3.99). 04/2011; 49(6):2222-9. DOI: 10.1128/JCM.01123-10
Source: PubMed


Amplified fragment length polymorphism (AFLP) was employed as a genetic analysis tool for the study of the genetic relatedness
of Mycobacterium avium subsp. paratuberculosis isolates harvested from bovine fecal samples and from bovine or human tissues. This analysis revealed genetic differences
between these two isolate types that were confirmed through cluster analysis. Dendrogram analysis separated these two isolate
types based on the isolation scheme (tissue-associated versus fecal M. avium subsp. paratuberculosis isolates). Further sequence analysis of unique genetic regions from each isolation type revealed no genetic sequence differences.
However, Clustal DNA alignments identified AFLP restriction enzyme sites that were undigested in the tissue-associated isolates.
AFLP analysis also disclosed that the same AFLP restriction sites were digested in all of the fecal isolates. Sequence analysis
further revealed a consensus sequence upstream of the undigested restriction sites for possible methyltransferase recognition
in the tissue-associated M. avium subsp. paratuberculosis isolates.

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