Characterization of epithelial V-like antigen in human choroid plexus epithelial cells: Potential role in CNS immune surveillance

Department of Neurology and Program in Cellular and Molecular Pathology, University of Wisconsin School of Medicine and Public Health, Madison, WI 53706, USA.
Neuroscience Letters (Impact Factor: 2.03). 03/2011; 495(2):115-20. DOI: 10.1016/j.neulet.2011.03.051
Source: PubMed


Prior work demonstrated that immune surveillance of the brain occurs primarily through the blood-cerebrospinal (CSF) fluid barrier rather than the blood-brain barrier endothelium. Recently, we identified epithelial V-like antigen (EVA), an immunoglobulin-like adhesion molecule, as a regulator of blood-CSF barrier integrity in a mouse model. Here we characterized EVA expression and function in human choroid plexus epithelial cells and analyzed its role in CD4 T lymphocyte adhesion. In human choroid plexus epithelial cells and a subset of CD4 T lymphocytes, EVA is expressed at high levels. Epithelial adhesion of T lymphocytes is inhibited by a blocking monoclonal antibody that recognizes EVA. T cell adhesion elicits calcium flux in choroid plexus epithelial cells that also can be blocked by an EVA-specific antibody. EVA-positive cell-cell contacts between epithelial and T cells are associated with increased complexity of cytoskeletal epithelial morphology. These results demonstrate that EVA is expressed in human choroid plexus epithelial cells and CD4 T lymphocytes and regulates CD4+ T lymphocyte adhesion to human choroid plexus epithelial cells in vitro. These data suggest a novel mechanism to regulate CNS immune surveillance.

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    • "Another difference is that kolmer cells are only present in the adult CP (Strazielle and Ghersi-Egea, 2000). Given the immunologic complexity of the stroma, much of the cell diversity is driven by immunologic insult, or CNS injury (Mitchell et al., 2009; Wojcik et al., 2011; Hasegawa-Ishii et al., 2013; Shechter et al., 2013; Szmydynger-Chodobska et al., 2013). Previous analysis in zebrafish failed to demonstrate presence of unlabelled cells in the mCP of transgenics suggesting that all cell lineages except vasculature derive from GFP-positive cells (Garcia-Lecea et al., 2008). "
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    • "Cells should be left undisturbed for at least 16 hours, then Epithelial Cell Medium supplemented with 10% FCS, antibiotics, epidermal growth factor and insulin should be added; all of these reagents are available from the supplier of this cell line. However, in some studies, these cells were grown successfully in the medium that included 2% FCS [30]. When cells attach they display polygonal shaped sheets of neighboring cells. "
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