MicroRNAs: Allies or Foes in Erythropoiesis?

Division of Hematology, Department of Medicine, University of Washington, Seattle, Washington, USA.
Journal of Cellular Physiology (Impact Factor: 3.84). 01/2012; 227(1):7-13. DOI: 10.1002/jcp.22729
Source: PubMed


MicroRNAs are small non-coding RNAs that negatively regulate gene expression through mRNA degradation or translational repression. It is becoming increasingly recognized that miRNAs play central roles in almost all cellular processes, and especially during development. The function of miRNAs in hematopoiesis, including erythropoiesis, is beginning to be elucidated. In this review, we will focus on what is known about miRNA function in various aspects of erythropoiesis and red cell physiology. J. Cell. Physiol. 227: 7–13, 2012.

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Available from: Thalia Papayannopoulou
    • "In granulocytes of PV patients, the expression of miR-182 and let-7a is increased and decreased, respectively. In platelets of these patients, miR-26b is increased, and miR-30b, miR-30c and miR-150 are decreased in their reticulocytes (Bruchova et al., 2008; Byon and Papayannopoulou, 2012). The role of these miRNAs in pathogenesis of this disease is still not well established, and requires further investigations. "
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    ABSTRACT: MicroRNAs (miRNAs) are 19-24 nucleotide non-coding ribonucleic acids binding DNA or RNA and controlling gene expression via mRNA degradation or its transcription inhibition. Erythropoies is a multi step differentiation process of erythroid progenitors to nucleate red blood cells. Maturation, proliferation and differentiation of red blood cells is affected by erythroid factors, signaling pathways in niche of hematopoietic cells, transcription factors as well as miRNAs. Expression of different types of miRNAs during erythroid development provides a background for the study of these molecules to control erythroid differentiation and maturation as well as their use as diagnostic and prognostic markers to treat erythroid disorders like thalassemia, sickle cell disease and erythrocyte enzyme deficiencies. In this paper, with reference to biosynthesis of miRNAs, their function in normal and anemic erythropoiesis has been investigated. The target molecule of each of these miRNAs has been cited in an attempt to elucidate their role in erythropoiesis. © 2015, Higher Education Press and Springer-Verlag Berlin Heidelberg.
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    • "MicroRNAs (miRNA) are 18–24 nucleotides single-stranded nonprotein-coding RNAs that function primarily as gene repressors by binding to the target messenger RNAs to regulate gene expression [14] [15] [16]. There is growing evidence that miRNAs regulate hematopoiesis in both hematopoietic stem cells and committed progenitor cells [17] [18] [19] [20] [21] [22] [23] [24] [25]. Deregulated miRNA profiles have been reported in MPN patients during in vitro erythroid differentiation as well as in peripheral blood granulocytes, platelets, and reticulocytes [26] [27]. "
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    ABSTRACT: Polycythemia vera (PV) and essential thrombocythemia (ET) are the two most common myeloproliferative neoplasms. The same JAK2(V617F) mutation can be found in both disorders and is able to recapitulate many of the phenotypic abnormalities of these diseases in the murine models. The disease phenotype is also influenced by other unknown genetic or epigenetic factors. MicroRNAs (miRNA) are 18-24 nucleotides single-stranded non-protein-coding RNAs that function primarily as gene repressors by binding to their target messenger RNAs. We performed miRNA expression profiling by oligonucleotide microarray analysis in purified peripheral blood CD34+ cells from eight JAK2(V617F)-positive PV patients and six healthy donors. A quantitative reverse-transcription polymerase chain reaction assay was used to verify differential miRNA expression. Since erythrocytosis is the only feature that distinguishes PV from ET, we also compared specific miRNA expression in the nucleated erythroid cells directly descended from the early erythroid progenitor cells of PV and ET patients. Our data indicate that significant miRNA deregulation occurs in PV CD34+ cells and confirm a genetic basis for the gender-specific differences that characterize PV with respect to miRNA. The results of our study also suggest that deregulated miRNAs may represent an important mechanism by which the PV erythrocytosis and ET thrombocytosis phenotypes are determined.
    Full-text · Article · Dec 2012 · Blood Cells Molecules and Diseases
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    • "Recent research has highlighted the function of miRNAs in erythropoiesis and in hemoglobin synthesis. [21] [22] This study investigates whether circulating miRNAs can be used as biomarkers for an erythropoiesis stimulating agent (C.E.R.A.) abuse. The purpose was to highlight a difference in the expression of specific miRNAs in plasma samples after C.E.R.A. injection. "
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    ABSTRACT: MicroRNAs (miRNAs) are small, non-protein coding transcripts involved in many cellular and physiological mechanisms. Recently, a new class of miRNA called ‘circulating miRNAs’ was found in cell-free body fluids such as plasma and urine. Circulating miRNAs have been shown to be very stable, specific, and sensitive biomarkers. In this paper, we investigate whether circulating miRNAs can serve as biomarkers for erythropoiesis-stimulating agent abuse. To this end, we analyzed miRNA levels in plasma by miRNA microarrays and quantitative real-time polymerase chain reaction (PCR). Plasma samples are derived from a clinical study with healthy subjects injected with erythropoiesis-stimulating agent (C.E.R.A.). Based on microarray results, we observed a significant difference in the levels of miRNAs in plasma after C.E.R.A. injection. We demonstrated that a specific miRNA, miR-144, exhibit a high increase that lasts 27 days after C.E.R.A. stimulation. Considering the fact that miR-144 is an essential erythropoiesis agent in different organisms, these findings suggest the possibility of using miR-144 as a sensitive and informative biomarker to detect C.E.R.A. abuse. Copyright
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