Guidelines for the measurement of BCR-ABL1 transcripts in chronic myeloid leukaemia

Department of Haematology,Imperial College Academic Health Science Centre, Hammersmith Hospital, Du Cane Rd., London, UK.
British Journal of Haematology (Impact Factor: 4.71). 03/2011; 153(2):179-90. DOI: 10.1111/j.1365-2141.2011.08603.x
Source: PubMed


Molecular testing for the BCR-ABL1 fusion gene by real time quantitative polymerase chain reaction (RT-qPCR) is the most sensitive routine approach for monitoring the response to therapy of patients with chronic myeloid leukaemia. In the context of tyrosine kinase inhibitor (TKI) therapy, the technique is most appropriate for patients who have achieved complete cytogenetic remission and can be used to define specific therapeutic milestones. To achieve this effectively, standardization of the laboratory procedures and the interpretation of results are essential. We present here consensus best practice guidelines for RT-qPCR testing, data interpretation and reporting that have been drawn up and agreed by a consortium of 21 testing laboratories in the United Kingdom and Ireland in accordance with the procedures of the UK Clinical Molecular Genetics Society.

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Available from: Gareth Gerrard
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    • "RT-qPCR is an important tool that may assist in the understanding of the molecular events underlying human diseases, but it also offers a method for measuring biomarkers for the identification and stratification of a range of diseases [32, 84]. Studies have reported applying RT-qPCR for the identification of micrometastases or minimal residual disease in colorectal cancer [85], neuroblastoma [86], prostate cancer [87] and leukaemia [88]. It has been used to distinguish different types of lymphoma [89], for the analysis of cellular immune responses in the peripheral blood [90, 91], for the detection of bacterial [92] and viral [93] RNA signatures in clinical samples and for monitoring the response of human cancer to treatment [13]. "
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    • "BCR - ABL1 transcripts were measured in whole blood for minimal residual disease monitoring using reverse transcrip - tion qPCR ( RT - qPCR ) using ABL1 as a reference gene , as reported previously ( Foroni et al , 2011 ; Gerrard et al , 2011 ) . SLC22A1 and BCR - ABL1 transcript levels , as well as those of two control genes : beta2 - microglobulin ( B2M ) and beta - glucuronidase ( GUSB ) , were quantified using pre - treatment diagnostic cDNA from whole blood leucocytes by RT - qPCR using a Taqman system on a StepOnePlus ( "
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