Three-Dimensional Chemical Patterns for Cellular Self-Organization

Department of Chemical and Biomolecular Engineering, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.
Angewandte Chemie International Edition (Impact Factor: 11.26). 03/2011; 50(11):2549-53. DOI: 10.1002/anie.201007107
Source: PubMed


Falling into line: A method for the precise generation of durable 3D chemical patterns within stationary media was used to direct the chemotactic self-organization of living cells. A container with carefully positioned slits, through which a chemoattractant (yellow) can diffuse, controls the self-organization of Escherichia coli cells (green ellipsoids) expressing green fluorescent protein into a helical pattern (see fluorescence images).

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