Antioxidant Activity of β-Carotene Compounds in Different in Vitro Assays

Abstract

β-Carotene (BC) is the most abundant carotenoid in human diet, almost solely as (all-E)-isomer. Significant amounts of (Z)-isomers of BC are present in processed food as well as in mammalian tissues. Differences are described for the activity of various BC isomers in forming retinal and protecting against cancer and cardiovascular diseases. Eccentric cleavage of BC leads to degradation products such as carotenals. A variety of negative consequences were published for the non-vitamin A active BC metabolites, such as inducing the carcinogenesis of benzo[a]pyrene, impairing mitochondrial function, or increasing CYP activity. To increase the knowledge on the antioxidant activity, a variety of BC isomers and metabolites were tested in various in vitro assays.
In the present study, no ferric reducing activity (FRAP assay) was observed for the BC isomers. Between the major BC isomers (all-E, 9Z, and 13Z) no significant differences in bleaching the ABTS●+ (αTEAC assay) or in scavenging peroxyl radicals (ROO●) generated by thermal degradation of AAPH (using a chemiluminescence assay) were detected. However, the (15Z)-isomer was less active, maybe due to its low stability. The degradation to β-apo-carotenoids increased FRAP activity and ROO● scavenging activity compared to the parent molecule. Dependence on chain length and character of the terminal function was determined in αTEAC assay with following order of increasing activity: β-apo-8’-carotenal < β-apo-8’-carotenoic acid ethyl ester < 6’-methyl-β-apo-6’-carotene-6’-one (citranaxanthin). The results indicate that BC does not lose its antioxidant activity by degradation to long chain breakdown products.

Full text

Molecules 2011, 16, 1055-1069; doi:10.3390/molecules16021055
molecules
ISSN 1420-3049
www.mdpi.com/journal/molecules
Article
Antioxidant Activity of β-Carotene Compounds in Different in
Vitro Assays
Lars Mueller and Volker Boehm *
Institute of Nutrition, Friedrich Schiller University Jena, Dornburger Straße 25-29, 07743 Jena,
Germany
* Author to whom correspondence should be addressed; E-Mail: volker.boehm@uni-jena.de;
Tel. +49(0)3641-949633; fax: +49(0)3641-949702
Received: 17 December 2010; in revised form: 13 January 2011 / Accepted: 18 January 2011 /
Published: 25 January 2011
Abstract: β-Carotene (BC) is the most abundant carotenoid in human diet, almost solely as
(all-E)-isomer. Significant amounts of (Z)-isomers of BC are present in processed food as
well as in mammalian tissues. Differences are described for the activity of various BC
isomers in forming retinal and protecting against cancer and cardiovascular diseases.
Eccentric cleavage of BC leads to degradation products such as carotenals. A variety of
negative consequences were published for the non-vitamin A active BC metabolites, such
as inducing the carcinogenesis of benzo[a]pyrene, impairing mitochondrial function, or
increasing CYP activity. To increase the knowledge on the antioxidant activity, a variety of
BC isomers and metabolites were tested in various in vitro assays.
In the present study, no ferric reducing activity (FRAP assay) was observed for the BC
isomers. Between the major BC isomers (all-E, 9Z, and 13Z) no significant differences in
bleaching the ABTS
●+
(αTEAC assay) or in scavenging peroxyl radicals (ROO
●
) generated
by thermal degradation of AAPH (using a chemiluminescence assay) were detected.
However, the (15Z)-isomer was less active, maybe due to its low stability. The degradation
to β-apo-carotenoids increased FRAP activity and ROO
●
scavenging activity compared to
the parent molecule. Dependence on chain length and character of the terminal function
was determined in αTEAC assay with following order of increasing activity: β-apo-8’-
carotenal < β-apo-8’-carotenoic acid ethyl ester < 6’-methyl-β-apo-6’-carotene-6’-one
(citranaxanthin). The results indicate that BC does not lose its antioxidant activity by
degradation to long chain breakdown products.
OPEN ACCESS

Molecules 2011, 16
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Keywords: β-carotene isomers; β-apo-carotenoids; ferric reducing; ABTS bleaching;
peroxyl radical scavenging
Introduction
Carotenoids are a widespread group of naturally occurring fat-soluble colorants. In developed
countries, 80-90% of the carotenoid intake comes from fruit and vegetable consumption. Of the more
than 700 naturally occurring carotenoids identified thus far, approx. 50 are present in the human diet
and can be absorbed and metabolized by the human body [1]. However, only six of them (β-carotene,
β-cryptoxanthin, α-carotene, lycopene, lutein and zeaxanthin) account for more than 95% of total
blood carotenoids. β-Carotene (BC) is a naturally occurring orange-colored carbon-hydrogen
carotenoid, abundant in yellow-orange fruits and vegetables and in dark green, leafy vegetables [2]. It
is also the most widely distributed carotenoid in foods [3]. BC undergoes trans (E) to cis (Z)
isomerization [4], whereas the (all-E)-form is the predominant isomer found in unprocessed carotene-
rich plant foods [5;6]. Food processing or long-term storage of carotenoid-rich vegetables can lead to
degradation and/or isomerization of carotenoids [1;7]. Although low concentrations are found in
circulating human serum, BC (Z)-isomers are present in human tissues where it is expected to exert
their biological function(s) [8]. Significant amounts of (9Z)-, (13Z)-, and (15Z)-isomers of BC were
found in liver, kidney, adrenal gland and testes up to 25% of the total BC, whereas in human serum
(all-E)-BC was the dominant isomer with 95% of the total BC amount [9]. Chemical structures of the
main BC isomers found in food and human tissues are shown in Figure 1.
Figure 1. Structures of analyzed β-carotene (BC) isomers and metabolites.
BC metabolites R= short name
β-apo-8’-carotenal CHO BC-8’-CHO
β-apo-8’-carotenoic acid ethyl ester COOCH
2
CH
3
BC-8’-COOEt
6’-methyl-β-apo-6’-carotene-6’-one (citranaxanthin) CH=CH–COCH
3
BC-6’-COMe
15
9
13
1
R
(all-E)-BC
(9Z)-BC
(13Z)-BC
(15Z)-BC
BC metabolites
1
15
8

Molecules 2011, 16
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Nutrition has a significant role in the prevention of many chronic diseases such as cardiovascular
diseases (CVD), cancers, and degenerative brain diseases [10]. The consumption of food-based
antioxidants like BC seems to be useful for the prevention of macular degeneration and cataracts [11].
Numerous epidemiological studies have suggested an inverse relationship between intake of BC, fruits
and vegetables, particularly raw fruits and vegetables and dark green, leafy and cruciferous vegetables,
and the risk of oesophageal adenocarcinoma and Barrett's oesophagus [12]. Additionally, several
studies have observed a protective effect of BC from foods, along with a diet rich in fruits and
vegetables, on liver carcinogenesis and lung disease [13;14]. BC has potential antioxidant biological
properties due to its chemical structure (see Figure 1) and interaction with biological membranes [15].
It is well-known, that BC quenches singlet oxygen with a multiple higher efficiency than α-tocopherol.
[16]. In addition, it was shown that (Z)-isomers of BC possess antioxidant activity in vitro [17-19].
In contrast, three large BC intervention trials: the β-Carotene and Retinol Efficacy Trial (CARET),
the Alpha-Tocopherol Beta-Carotene Cancer Prevention Study (ATBC), and the Physician's Health
Study (PHS) have all pointed to a lack of effect of synthetic BC in decreasing cardiovascular disease
or cancer risk in well-nourished populations up to increased lung cancer incidence and mortality in
smokers [14;20;21].
In vertebrates, BC is converted into two molecules of retinal, in a reaction catalyzed by β,β-
carotene-15,15’-monooxygenase (BCMO I), like other provitamin A carotenoids too [22]. Of the 50
different carotenoids that can be metabolized into vitamin A, BC has the highest provitamin A activity
[2]. The formed retinal is further metabolized to the vitamin A derivatives retinoic acid (RA) and
retinol. The provitamin A activity of (Z)-isomers is much lower than that of (all-E)-BC. (9Z)-BC has a
relative bioconversion to retinol of 38%, (13Z)-BC 53% whereas the (all-E)-form is 100% [23].
Besides being essential for vision, RA is a major signal pathway controlling molecule which regulates
a wide range of biological processes. RA is the ligand of two classes of nuclear receptors, the retinoic
acid receptors (RARs) and the retinoid X receptors (RXRs). (all-E)-BC is a precursor of (all-E)-RA,
which preferentially binds to RARs, whereas (9Z)-BC is a precursor of (9Z)-RA – the preferred ligand
for RXRs [24].
In addition to this central cleavage pathway, an eccentric cleavage was proposed in healthy
mammals after incubation of BC with liver, kidney and lung homogenate of rats, ferrets, and monkeys
[25]. By stepwise oxidation from one end of the polyene chain a sequence of β-apo-carotenal
derivatives were presumably formed, e.g. β-apo-8’-carotenal (shown in Figure 1). The formed
aldehydes were further cleaved to short-chain carbonyl compounds, or converted to β-apo-carotenol,
β-apo-carotenoic acids or their esters, or oxidized to retinoic acid by β-oxidation pathways [26;27].
The three apo-carotenoids studied herein are used as colorants in animal feed and human food. β-apo-
8’-Carotenal and β-apo-8’-carotenoic acid ethyl ester are present in some fruits and vegetables, though
in low amounts [28], and were recently detected in human plasma [27].
In addition to the enzymatic cleavage of BC in mammalian metabolism, free radical attack on BC
results in the formation of high amounts of cleavage products. For instance, β-apo-8’-carotenal and 6’-
methyl-β-apo-6’-carotene-6’-one (citranaxanthin), shown in Figure 1, were identified in minor
amounts in intestinal extracts of vitamin A deficient rats [29]. The results of Allija et al. [30] indicate a
genotoxic potential of BC cleavage products at physiologically relevant levels of BC and its
breakdown products. In contrast, BC itself did not induce cytotoxic or genotoxic effects. Furthermore,

Molecules 2011, 16
1058
when BC was supplemented to primary hepatocytes a dose-dependent increase of cleavage products
was observed accompanied by increasing genotoxicity [30]. The authors speculated that these results
provide strong evidence that BC breakdown products are responsible for the occurrence of
carcinogenic effects found in the Alpha-Tocopherol Beta-Carotene Cancer prevention (ATBC) study
and the β-Carotene and Retinol Efficacy (CARET) Trial.
In contrast to the physiologically relevant properties, such as influencing cellular signal pathways,
gene expression or induction of detoxifying enzymes, the knowledge on antioxidant potential of BC
compounds is scarce. Therefore, the aim of the study was to investigate both BC isomers and some
non-retinoic metabolites on their antioxidant activity in various in vitro assays, compared to another
nutritionally relevant substance – vitamin E (α-tocopherol).
Results and Discussion
It has been known for many years that carotenoids undergo ‘‘bleaching’’ i. e., lose their color, when
exposed to radicals or to oxidizing species. This process involves interruption of the conjugated double
bond system either by cleavage or by addition to one of the double bonds. Cleavage can be detected by
characterizing the products that are formed, which are frequently carbonyls or epoxides [2]. In the
present study, four isomers and three metabolites of β-carotene (BC) were analyzed on their
antioxidant activity in three different in vitro assays. There are at least three possible mechanisms for
the reaction of carotenoids with radical species. They include (1) radical addition; (2) electron transfer
to the radical; or (3) allylic hydrogen abstraction [2].
The ability of BC and its degradation products to undergo single electron transfer-based reactions
(SET) was utilized in the analysis of ferric reducing (FRAP) and ABTS
●+
bleaching (αTEAC) activity.
Electron transfer reactions have been reported, resulting in the formation of a carotenoid cation radical
(CAR
●+
) [31]. Such a cationic radical of BC or its metabolites is entirely conceivable in the reactions
with the ferric ion or the synthetic ABTS
●+
.
In the αTEAC assay, the investigated (all-E)-BC and its (Z)-isomers showed 3-times higher
ABTS
●+
bleaching activity than α-tocopherol [Figure 2(A)]. The results of Böhm et al. showed an
antioxidant activity of the BC isomers dissolved in n-hexane, marginal higher than that of the
calibration compound Trolox
®
. This hydrophilic analogue of α-tocopherol was dissolved in PBS [19].
In the present study, the reference compound α-tocopherol was dissolved in n-hexane to be more
comparable to the reaction conditions used for the carotenoids. The differences in the reference
compound used and in the reaction conditions might have caused the different TEAC values of BC. To
date, published results on antioxidant activity of BC isomers in vitro differ due to the use of different
test systems. Often (9Z)-BC was more effective than its (all-E)-isomer [17;18;32]. In contrast, there
are also investigations under identical conditions which support our results. The studies of Böhm and
colleagues showed that the ABTS
●+
bleaching activity of BC isomers is independent from position of
the cis-double bond [19]. No significant dependence (p > 0.05) of the position of the cis-double bond
was observed between (all-E)-, (9Z)-, and (13Z)-BC (approx. 3 mol α-TE/mol) in our investigations.
However, (15Z)-BC displayed a 20% lower activity (2.5 mol α-TE/mol) in this assay (p < 0.05). The
advanced hindrance between the steric demanding bicyclic carotenoid molecule with a centered cis-
double bond and the similarly demanding oxidizing agent ABTS
●+
might have caused this lower

Molecules 2011, 16
1059
activity. The relation of steric demand of ABTS and carotenoids was demonstrated several times
[33;34]. Ascorbic acid and phenolic antioxidants like flavonoids, phenolic acids (hydroxylated benzoic
acids and cinnamic acids) and tocopherols excite their antioxidant potential by hydroxyl groups at the
outer part of the molecule reacting with radicals combined with a conjugated double bond system in
vicinity [33;34]. Consequently, a resonance-stabilized radical is formed [37;38]. However, the reactive
part of carotenes, like lycopene, α- and β-carotene, is the conjugated polyene chain in the center of the
molecule [39]. This fact makes it difficult for steric demanding oxidants to interact with the carotenoid,
especially with the bicyclic structures of β-carotene. The results obtained in the FRAP assay, described
below, support this hypothesis. Additionally, (15Z)-BC is the BC isomer with the lowest stability
[40;41] investigated in the present work, due to the higher potential energy of its cis-bond. This may
have led to a degradation of this isomer during analysis, and consequently a lower antioxidant activity
was determined.
Figure 2. Antioxidant activities (mean ± SD) of β-carotene (BC) isomers and metabolites (at
10 µM) determined by αTEAC (A), FRAP (B) and CL (C) assay with respect to α-tocopherol (α-
TE, α-tocopherol equivalents); different superscript letters denote significant differences
(ANOVA, post-hoc Student-Newman-Keuls, p < 0.05). For abbreviations see Figure 1.
0
1
2
3
4
antioxidant activity [mol α-TE/mol]
a
a
a
c
b
b
d
A
0
0.5
1
1.5
2
antioxidant activity [mol α-TE/mol]
a
a
a
b
a
b
b
B
0
5
10
15
20
25
30
antioxidant activity [mol α-TE/mol]
a
a
a
c
b
cc
C

Molecules 2011, 16
1060
Transition metals, such as iron (III) and copper (II), play an important role in the oxidation of LDL
in vitro as well as in vivo, leading to atherosclerosis [42]. BC and other carotenoids have potential
antioxidant properties [15], and they were found to be incorporated into LDL particles. However, in
our in vitro studies, none of the BC isomers showed ferric reducing activity [Figure 2(B)] under the
used conditions, which support the findings of Pulido and co-workers [43]. This may be due to the
circumstance that the ferric ion is incorporated into the steric demanding di-tripyridyltriazine (TPTZ)
complex, which was first applied by Benzie and Strain [44]. Our recently published findings using α-
carotene, β-carotene, lycopene and a variety of xanthophylls in the FRAP assay supports this
hypothesis. It was shown, that lycopene with its acyclic polyene structure showed FRAP activity. The
insertion of a hydroxyl function into bicyclic carotenes (leading to e.g. β-cryptoxanthin and zeaxanthin)
induced the activity to reduce ferric ions using the TPTZ complex method [34]. The buckle in cis-
isomers of BC, which may open the molecule to be more assailable to react with large steric
demanding oxidants, such as ferric di-TPTZ in the FRAP assay, in our case, did not influence the
activity of BC.
The noxious effects of an uncontrolled production of oxygen- and nitrogen-centered radicals (ROS,
RNS) are amplified by chain reactions (autoxidations), sustained mainly by peroxyl radicals (ROO
●
),
that oxidize and alter essential biomolecules such as lipids, lipoproteins, proteins and nucleic acid
[45;46]. Krinsky and Johnson [2] proposed that ROO
●
might add to any place across the polyene chain
of a carotenoid, resulting in the formation of a resonance-stabilized, carbon-centered radical (ROO-
CAR
●
). Unfortunately, radical-carotenoid reaction products or stabilized carotenoid radicals were not
detected in vivo to date. Additionally, ROO
●
can abstract an allylic hydrogen atom at the periphery of
the carotenoid, in the case of BC at the 4- and 4’-position [47]. In the present study, the ROO
●
were
formed by thermal degradation of AAPH at 37 °C. The analyzed (all-E)-form of BC presented a ROO
●
scavenging activity, being approx. 20-times higher than that of α-tocopherol [Figure 2(C)]. Hence, β-
carotene and its isomers could play a role in the endogenous antioxidant defense system despite their
lower concentrations found in human tissues compared to tocopherols. The high scavenging rate found
in the present studies supports our recent observations and that of other research groups using typical
synthetic ROO
●
generating azo-initiators such as AAPH, AMVN or AIBN [34;48-51]. The insertion of
a cis-double bond at C9 or C13 did not change the antioxidant activity of BC (p > 0.05), whereas
(15Z)-BC (9.5 mol α-TE/mol) was half as active (p < 0.05) as (all-E)-BC (18.8 mol α-TE/mol)
probably caused by oxidative degradation during the analysis as explained for the αTEAC assay above.
Within the investigated BC metabolites, 6’-methyl-β-apo-6’-carotene-6’-one showed the highest
ABTS
●+
bleaching activity [Figure 2(A)], approx. 4-times higher than α-tocopherol and significantly
(p < 0.05) higher than its parent molecule (all-E)-BC (3.0 mol α-TE/mol), which has the same number
of conjugated double bonds (CDB) in the polyene chain. The degradation of BC to β-apo-8’-carotenal
and its related carotenoic acid ester led to a significant (p < 0.05) decrease of CDB and therefore to a
decrease of αTEAC activity. The β-apo-8’-carotenal (1.4 mol α-TE/mol) was only 40% more active
than α-tocopherol (1 mol α-TE/mol) and only half as active as (all-E)-BC (3.0 mol α-TE/mol), due to
its shorter polyene chain system and the electron-withdrawing effect of the carbonyl function. This
circumstance causes a higher ionization potential of β-apo-8’-carotenal (4.676 eV) compared to BC
(4.414 eV) calculated by Galano [52]. Ionization potentials of compounds are important in SET-based
assays such as αTEAC and FRAP assay. In contrast, the change in the terminal function to a

Molecules 2011, 16
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carboxylic acid ester with equal chain length led to an comparable activity to (15Z)-BC (2.5 mol α-
TE/mol), possibly caused by an inductive effect inserted by esterification of the carbonyl function.
Surprisingly, the breakdown of BC to its metabolites caused a significant increase (p < 0.05) of the
ferric reducing activity, which supports the hypothesis, that the existence of two non-substituted
β-ionone rings has caused the absent FRAP activity of BC. The cleavage of BC to its metabolites,
forming a structure with only one β-ionone ring and an oxygenated functional group at the opposite
side, led to a significant increase of the ferric reducing activity. The three BC metabolites showed
FRAP values being 25% higher than that of α-tocopherol, however, without any significant differences
(p > 0.05) concerning the length of the conjugated chain or terminal function [Figure 2(B)] of
the compounds.
The three investigated BC breakdown products were highly efficient in preventing luminol
oxidation (23.8-25.1 mol α-TE/mol). The degradation of BC to these metabolites led to a significant
(p < 0.05) increase in the ROO
●
scavenging activity of approx. 25% [Figure 2(C)]. A significant
dependence on chain length or carbonyl related function was not observed (p > 0.05). ROO
●
can
abstract a hydrogen atom at each position of the polyene chain [2]. In consequence, the type of
terminal function has only significant influence on the reaction between carotenoid and radical if the
conjugated double bond system expands. The increase of the activity to scavenge ROO
●
radicals by
insertion of carbonyl functions into the polyene molecule was described several times for BC and its
related ketocarotenoids echinenone, canthaxanthin, and astaxanthin [34;49;53]. Carotenoids such as
BC can prevent the propagation phase of lipid peroxidation. As known from fatty acid oxidation, the
final result of this reaction is degradation of the whole molecule into small polar products. BC should
be regarded as peroxidation substrate as well as antioxidative compound [17]. Our studies show that
the long-chained non-enzymatic metabolites such as β-apo-8’-carotenal are able to act as substrate in
peroxidation and could protect fatty acids from oxidation, too. However, due to the very low amounts
of BC metabolites found in vivo compared to tocopherols and carotenoids, BC metabolites might be
not of relevance for the antioxidant defense system in human organism.
As stressed by Huang et al. [54], no single method is adequate for evaluating the antioxidant
activity of single compounds or antioxidant capacity of foods or biological samples. Methods based on
different mechanistic principles can yield widely diverging results. A variety of methods must be used.
In the present study, two different principles were used: αTEAC and FRAP assay measures reducing
activity, whereas CL determined ROO
●
scavenging activity. Standardization is needed by a calculation
of the results achieved in the three assays. A simple mathematical treatment is not indicated, because
the CL assay gave much higher values due to the low activity of α-tocopherol in this assay. To give no
substance in any assay undue preponderance, calculating a global antioxidant activity as a weighted
average of the results is necessary [55]. First, the antioxidant activity of the compound detected in the
specific method was divided by the average activity of the whole set of compounds by the same
method. Afterwards, the calculated values of the specific compounds in each assay were summed and
divided by the number of assays used (three in our case). The resulting weighted averages of each
compound are given in Table 1 (last column).

Molecules 2011, 16
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Table 1. Antioxidant activities (mol α-TE/mol) of β-carotene isomers and metabolites standardized
with respect to α-tocopherol measurement.
Compound αTEAC FRAP CL
Weighted
avera
g
e
α-tocopherol 1.0 1.0 1.0 0.7
β-carotene
isomers
(all-E)-β-carotene 3.0 0.0 18.8 0.8
(9Z)-β-carotene
3.1 0.0 19.8 0.8
(13Z)-β-carotene
3.1 0.0 19.6 0.8
(15Z)-β-carotene
2.5 0.0 9.5 0.5
β-carotene
metabolites
β-apo-8’-carotenal
1.4 1.3 23.8 1.3
β-apo-8’-carotenoic acid ethyl ester
2.5 1.3 25.1 1.5
6’-methyl-β-apo-6’-carotene-6’-one
3.7 1.3 24.5 1.7
Average
2.5 0.6 17.8
The right-hand column shows the weighted averages (mol α-TE/mol) obtained by (1) dividing the
antioxidant activity of each compound, as determined by the specified method, by the average
activity determined for the whole set of compounds by the same method (last row), (2) summing
the results of the three assays for the specific compound (αTEAC, FRAP, and CL), and (3) dividing
the sum by three.
On this basis, the four analyzed BC isomers showed antioxidant activities comparable to that of α-
tocopherol (0.5-0.8 mol α-TE/mol) due to the absent ferric reducing activity of BC-isomers, whereas
α-tocopherol displayed a poor CL value. Almost two-times higher activities were observed for the BC
breakdown products, with 6’-methyl-β-apo-6’-carotene-6’-one as the most active one (weighted
average of 1.7 α-TE/mol).
In addition to our findings on antioxidant activities of BC metabolites, the pro-oxidative effects
have to be kept in mind as well. β-apo-8’-Carotenal was shown to be a strong inducer of cytochromes
P4501A1 and 1A2 in rat liver, whereas BC itself was not active [56]. Induced cytochrome P450
enzymes could enhance the activation of carcinogens. Oxidative degradation products of BC could
also increase the binding rate of benzo[a]pyrene to DNA [57] and may impair mitochondrial function
[58-60]. And β-apo-8’-carotenal was shown to bound to 2’-deoxyguanosine in vitro [61]. In contrast,
various beneficial activities were demonstrated in vitro for oxidation products of non-provitamin A
carotenoids e.g. lycopene [62].
Conclusions
According to our knowledge this is the first study presenting antioxidant activity data of β-carotene
(BC) isomers and their metabolites using different types of in vitro assays. For the first time, BC
related compounds were compared based on their ABTS
●+
bleaching and ferric reducing activity, as
well as on their ROO
●
radical scavenging activity. All results were compared to the activity of α-
tocopherol, which is known as the most active chain breaking and major fat-soluble antioxidant in
human tissues. The activity of carotenoids to reduce ferric ions is an important property, because
transition metals play an important role in catalyzing LDL oxidation in vitro and in vivo, leading to

Molecules 2011, 16
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atherosclerosis. However, in the present study, ferric reducing activity was detected for BC metabolites,
but not for the different BC isomers. Additionally, scavenging activities of the investigated compounds
against ROO
●
generated by thermal degradation of AAPH were 10-25-times higher than that of α-
tocopherol. ROO
●
are important for the initiation of lipid peroxidation chain reactions in food as well
as in biological samples. All analyzed BC isomers showed 2.5-3-times higher activity in bleaching
ABTS
●+
than α-tocopherol. Dependence on the antioxidant activity from chain length and terminal
group of the β-apo-carotenoids was only observed in the activity of bleaching ABTS
●+
, but not in the
more in vivo relevant activities like reducing ferric and scavenging ROO
●
. The results of the different
assays were summarized by calculating a weighted average for each BC compound to get an overall
impression of the antioxidant potential. On this basis, the global antioxidant activity of the BC isomers
was comparable to that of α-tocopherol. The activity of breakdown products of BC was twice as high.
Experimental
General
2,2´-Azinobis(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), K
2
S
2
O
8
, and
2,4,6-tripyridyltriazine (TPTZ) were obtained from Sigma-Aldrich (Taufkirchen, Germany). 2,2´-
Azobis(2-amidinopropane) dihydrochloride (AAPH) was obtained from Acros Organics (Schwerte,
Germany). Luminol was purchased from Fluka (Buchs, Switzerland). DL-α-Tocopherol was purchased
from Calbiochem (Darmstadt, Germany) with a purity of 100% shown by GC. β-Carotene (BC)
isomers, 6’-methyl-β-apo-6’-carotene-6’-one (citranaxanthin), β-apo-8’-carotenal and β-apo-8’-
carotenoic acid ethyl ester were obtained from CaroteNature (Lupsingen, Switzerland) with a purity of
97-99% by HPLC. All solvents used, such as tert-butyl methyl ether (TBME) or dimethyl sulfoxide
(DMSO), were of HPLC grade. HPLC grade water (18 MΩ) was prepared using a Millipore Milli-Q
purification system (Millipore GmbH, Schwalbach, Germany). Buffer salts for phosphate buffered
saline (PBS), borax buffer and acetic acid buffer and all other chemicals were of analytical grade.
Equipment
An ABTS
●+
solution was prepared in phosphate buffered saline (PBS, 75 mM, pH 7.4) to measure
the activity of the BC compounds to bleach ABTS
●+
in the αTEAC (α-tocopherol equivalent
antioxidant activity) assay as described in several publications [19;33;63]. To determine the ferric
reducing antioxidant power (FRAP) of BC and its derivatives, a FRAP reagent was prepared as
recently described [63;64]. The analysis of the ROO
●
radical scavenging activity in a
chemiluminescence (CL) based assay followed the descriptions as published recently [61]. A luminol
solution in DMSO+borax buffer (80+20, v/v) as well as an AAPH solution in DMSO+PBS
(80+20, v/v) was prepared daily fresh, and cooled until analysis. Stock solutions of (all-E)-BC, its (Z)-
isomers and metabolites were prepared by dissolving the compounds in toluene+cylohexane (1+4, v/v)
to concentrations of 150 µmol/L. A 2.5 mmol/L α-tocopherol stock solution was prepared in ethanol.
All stock solutions were stored at -25 ± 2 °C. Prior to analysis, aliquots of the stock solutions were
transferred into reaction tubes and the solvent was removed under nitrogen at 30±1 °C in darkness.
The residues were immediately dissolved in n-hexane (for the use in FRAP and αTEAC assay) or tert-

Molecules 2011, 16
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butyl methyl ether (TBME)+DMSO (1+9, v/v) for the application in the CL assay. Concentrations of
the compounds were adjusted to 100 µmol/L by spectrophotometrical determination using the
absorptivity values (E
1 %, 1 cm
) at the specific wavelengths listed in Table 2.
Table 2. Absorptivity values at specific wavelength maxima in specific solvent, and
solvent used for stock solutions of analyzed β-carotene isomers and metabolites and α-
tocopherol [65-68].
Compound Solvent
Wavelength
(nm)
Absorptivity
value (E
1%,1 cm
)
Solvent used for
stock solution
(all-E)-β-carotene n-hexane 453 2592 T/CH (1+4, v/v)
(9Z)-β-carotene n-hexane 445 2550 T/CH (1+4, v/v)
(13Z)-β-carotene n-hexane 443 2090 T/CH (1+4, v/v)
(15Z)-β-carotene n-hexane 447 1820 T/CH (1+4, v/v)
β-apo-8’-carotenal ethanol 457 2640 ethanol
β-apo-8’-carotinoic acid
ethyl ester
cyclo-
hexane
446 2540 ethanol
6’-methyl-β-apo-6’-
carotene-6’-one
n-hexane 468 2745 T/CH (1+4, v/v)
DL-α-tocopherol ethanol 292 75.8 ethanol
T/CH, toluene+cyclohexane
The compounds were analyzed on FRAP and αTEAC activity in a V-530 spectrophotometer
(JASCO, Groß-Umstadt, Germany) using half-micro cuvettes (1.5 mL, polystyrene; Plastibrand,
Wertheim, Germany). A microplate reader FluoStar Optima (BMG Labtech, Offenburg, Germany)
was used to analyze ROO
●
radical scavenging activity in the CL assay. The antioxidant activity was
calculated using a dose-response curve for α-tocopherol (approx. 5-250 µM) in n-hexane (for αTEAC
and FRAP assay) or in TBME+DMSO (1+9, v/v) for CL assay, respectively [63]. The pure solvents
were used as blank in the specific assay. The antioxidant activity of BC and its metabolites in each
assay was calculated as mol α-tocopherol equivalents (α-TE)/mol compound.
Determination of antioxidant activity
αTEAC, FRAP and CL assay to assess the antioxidant activity of BC isomers and its metabolites
were done as described by our research group [34]. αTEAC assay was performed by mixing ABTS
●+
working solution with solutions of BC isomers, its metabolites or with α-tocopherol standard.
Thereafter, the mixture was completely transferred into cuvettes and centrifuged. Finally, the
absorbance of the lower phase (ABTS layer) was measured at 734 nm. To assess the FRAP activity of
these lipophilic compounds, solutions of α-tocopherol standard, BC isomer or metabolite were mixed
with FRAP reagent. After transferring the mixed solution into cuvettes, and subsequent centrifugation,

Molecules 2011, 16
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the absorbance of the aqueous layer was measured at 595 nm. To quantify the ROO
●
radical
scavenging activity of the BC compounds, a CL assay was performed, using luminol as CL dye and
AAPH as ROO
●
generator [69]. The assay was carried out in white 96-well Lumitrac micro plates
(Greiner Bio-One, Frickenhausen, Germany). Luminol solution (in DMSO+borax buffer), solution of
BC compound or α-tocopherol standard in TBME+DMSO (9+1, v/v), were combined in the wells of
the micro plate. After addition of AAPH solution, the instrument was started to record the
luminescence signals [34].
Statistics
All analyses were performed in triplicate at four different concentrations of each BC compound (1-
20 µmol/L). Differences of the antioxidant activity between (all-E)-β-carotene, its (Z)-isomers and its
metabolites were calculated using one way analysis of variance (ANOVA) with Student-Newman-
Keuls post-hoc procedure, with a level of significance at p < 0.05 (SPSS for Windows, version 18.0;
SPSS Inc., Chicago, IL).
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