Tail and fin rot disease occurred in Indian major carp, catla ( Catla catla ) and climbing perch, koi ( Anabas testudineus ) in fish farms located at two districts of Bangladesh. The affected fish showed lesion and erosion on the tail and fins. Approximately, 40% mortality was recorded in those farms. The present study was conducted to isolate and identify the bacterial pathogen causing the disease, to conduct artificial infection challenge for confirmation of the pathogen and to know the antibiotic sensitivity pattern of the isolates. Bacteria were isolated from the lesions of diseased fish on Cytophaga agar medium where they developed characteristic yellowish pigmented colonies. They were identified as Flavobacterium columnare based on biochemical characterization tests. All of the isolates were found to be highly virulent for carp fish ( Puntius gonionotus ) in artificial infection challenge experiment but, virulence for koi fish ( A. testudineus ) were found to be varied. These isolates exhibited sensitivity to antibiotics chloramphenicol, oxytetracycline, erythromycin, streptomycin, but some of them were resistant to sulphamethoxazole and all were resistant to gentamicin and cefradine.