Laceyella sediminis sp. nov., a thermophilic bacterium isolated from a hot spring

Biotechnology Research Center, Faculty of Life Science and Technology, Kunming University of Science and Technology (KUST), Kunming, PR China.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 02/2011; 62(Pt 1):38-42. DOI: 10.1099/ijs.0.028282-0
Source: PubMed


A thermophilic bacterium, designated strain RHA1(T), was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China, and was characterized by using a polyphasic approach. Based on its phenotypic and phylogenetic characteristics, strain RHA1(T) was affiliated to the genus Laceyella. The strain formed white aerial and yellow-white substrate mycelia, bearing single endospores on short sporophores. The cell-wall peptidoglycan contained meso-diaminopimelic acid. Whole-cell hydrolysates contained ribose and glucose. The major fatty acids were iso-C(15:0) (62.39%) and anteiso-C(15:0) (17.55%)(.) The predominant menaquinone was MK-9. The G+C content of the genomic DNA of strain RHA1(T) was 47.9 mol%. Based on DNA-DNA hybridization data, chemotaxonomic characteristics and differential physiological properties, strain RHA1(T) is considered to represent a novel species of the genus Laceyella, for which the name Laceyella sediminis sp. nov. is proposed; the type strain is RHA1(T) (=DSM 45263(T)=CCTCC AA 208058(T)).

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Available from: Wen-Jun Li, Jun 13, 2015
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    • "The genus Laceyella was proposed by Yoon et al. 2005 after a detailed polyphasic study and reclassification on the genus Thermoactinomyces [1]. At present the genus Laceyella has four recognized species namely; Laceyella putida [1], L. sacchari, type species of the genus Laceyella [1], Laceyella sediminis [2] and Laceyella tengchongensis [3]. Strain GS 1-1 is a Gram-positive bacteria and thermophilic bacteria. "
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    ABSTRACT: We report the 3.3-Mb draft genome of Laceyella sacchari strain GS 1-1, isolated from hot spring water sample, Chumathang, Leh, India. Draft genome of strain GS 1-1 consists of 3, 324, 316 bp with a G + C content of 48.8% and 3429 predicted protein coding genes and 75 RNAs. Geobacillus thermodenitrificans strain NG80-2, Geobacillus kaustophilus strain HTA426 and Geobacillus sp. Strain G11MC16 are the closest neighbors of the strain GS 1-1.
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    • "During our investigation of thermophilic micro-organisms in this geothermal field, three members of the family Thermoactinomycetaceae have been described in previous studies (Chen et al., 2012; Yu et al., 2012; Zhang et al., 2010), and Planifilum yunnanense, described by Zhang et al. (2007), was also isolated from this area. Here, we characterize another new member of the family Thermoactinomycetaceae on the basis of phylogenetic and polyphasic biochemical studies. "
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    • "(Lacey and Cross 1989; Yoon et al. 2005; Chen et al. 2011 "
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