Investigation of the effects of the chronic administration of some antihypertensive drugs on enzymatic and non-enzymatic oxidant/antioxidant parameters in rat ovarian tissue

Ministry of Health, Obstetrics and Gynecology Hospital, Iğdır, Turkey.
Gynecological Endocrinology (Impact Factor: 1.33). 11/2011; 27(11):895-9. DOI: 10.3109/09513590.2010.551564
Source: PubMed


In this study, effects of chronic antihypertensive drug (clonidine, methyldopa, amlodipine, ramipril, and rilmenidine) treatment on antioxidant-oxidant parameters were investigated in rat ovarian tissue.
Chronic drug administration for 30 days and at the end, biochemical examinations (total glutathione (tGSH), glutathione peroxidase (GPO), glutathione reductase (GR), glutathione s-transferase (GST), superoxide dismutase (SOD), nitric oxide (NO), catalase (CAT), malondialdehyde (MDA), and myeloperoxidase (MPO) analyses) were performed.
The levels of glutathione (GSH) and NO, and the activities of GPO, GR, GST, SOD, and CAT were measured the lowest in ramiprile group. Also in ramiprile group, the level of MDA and the activity of MPO was the highest.
We divided the drugs into four groups according to their biochemical side effect potentials in ovarian tissue: (I) Drugs which have no clear negative effect on ovarian tissue: clonidine, rilmenidine; (II) Drugs which have mild negative effect on ovarian tissue: methyldopa; (III) Drugs which have moderate negative effect on ovarian tissue: amlodipine; (IV) Drugs which have severe negative effect on ovarian tissue: ramipril. These data might be useful in the selection of the least toxic antihypertensive drug in pregnant and/or normal females.

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    • "In the experiment, clonidine (0.075 mg/kg), methyldopa (100 mg/kg), amlodipine (2 mg/kg), ramipril (2.5 mg/kg) and rilmenidine (0.5 mg/kg) were administrated to female rats by gastric gavage for 30 days, once a day (12). Distilled water was given to the healthy control group as vehicle, at the same time. "
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