Bovine Colostrum: Determination of Naturally Occurring Steroid Hormones by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Tiergesundheitsdienst Bayern e.V. , Senator-Gerauer-Strasse 23, 85586 Poing, Germany.
Journal of Agricultural and Food Chemistry (Impact Factor: 2.91). 02/2011; 59(4):1423-7. DOI: 10.1021/jf103751z
Source: PubMed


The aim of this study was to collect further data about levels of endogenous hormones in foodstuffs derived from animal production. Because of expected higher concentrations of especially estrogens in colostrum compared to other foodstuffs, our investigation focused on this matrix. For evaluation of endogenous steroid hormones in separated colostrum (fat and defatted fraction) and colostrum powder, the relevant free and conjugated estrogens (estradiol-17β, estradiol-17α, estrone, and estriol) androgens (androstendione, 19nor-androstendione, 19nor-testosterone-17β, 19nor-testosterone-17α, testosterone-17β, and testosterone-17α), and progesterone were determined by means of liquid chromatography-tandem mass spectrometry (LC-MS/MS). Upmost determined concentrations were found in the fat fraction, with 25.56 and 7.59 μg/L for estrone and androstendione, respectively. In defatted milk and colostrum powder, conjugated estrogens dominated, whereas total (free and conjugated) estrone (5.51 μg/L; 15.0 μg/kg) exceeded estradiol-17α (2.66 μg/L; 7.5 μg/kg) and estradiol-17β (2.28 μg/L; 3.3 μg/kg). Neither 19nor-steroids nor estriol were detected in colostrum fractions or processed colostrum.

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    • "of colostrum exceeds the concentrations published for other products , such as meat , milk and milk products ( Farke et al . 2011 ) . Besides , within the colostrum , the higher concentrations are found in the fat fraction , as it can be observed in Table 4 . As hormonal levels in colostrum are , in general , beyond those in other foodstuffs ( Malekinejad et al . 2006 ; Courant et al . 2008 ) , this kind of milk and its products should be strongly considered when "
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