Oxidative Stress Increases in Overweight Individuals Following an Exercise Test

Walter Reed Army Medical Center, 6900 Georgia Avenue, NW Washington, DC 20307, USA.
Military medicine (Impact Factor: 0.77). 12/2010; 175(12):1014-9. DOI: 10.7205/MILMED-D-09-00155
Source: PubMed


The objective of this study was to determine whether the Army Physical Fitness Test (APFT) causes oxidative stress and evaluate the impact of dietary antioxidant intake, fitness level, and body composition on changes in oxidative stress. Forty-seven overweight subjects were asked to perform an APFT. Creatine kinase (CK), C-reactive protein (CRP), glutathione peroxidase (GPX), and superoxide dismutase (SOD) were measured before, immediately after, and 24 hr postexercise. CK significantly increased immediately postexercise and at 24 hr postexercise. CRP and GPX significantly increased immediately postexercise, whereas SOD did not change significantly. Antioxidant intake, fitness level, and body composition were found to significantly influence changes in CK, GPX, and SOD after exercise. In conclusion, the APFT causes oxidative stress in overweight subjects. The associations between dietary antioxidants, fitness level, and body composition seen with each of the biomarkers provide support for future research in this area.

Full-text preview

Available from:
  • [Show abstract] [Hide abstract]
    ABSTRACT: To assess the impact of fitness status and physical activity on oxidative stress in prepubertal children, we measured selected biomarkers such as protein carbonyls (PC), lipid peroxidation products, and total nitrites, as well as the antioxidant system: total glutathione (TG), oxidized glutathione (GSSG), reduced glutathione (GSH), superoxide dismutase activity, and glutathione peroxidase. A total of 132 healthy children ages 7-12, at prepubertal stage, were classified into two groups according to their fitness level: low fitness (LF) and high fitness (HF). They were observed while engaged in an after-school exercise program, and a questionnaire was created to obtain information on their physical activity or sedentary habits. Plasma and red blood cells were obtained to analyze biomarkers. Regarding oxidative stress markers, the LF group and the sedentary group showed higher levels of TG and GSSG and a lower GSH/GSSG ratio than the HF group and the children engaged in physical activity. A negative association was found between PC and GSSG and TG and between TG and the GSH/GSSG ratio. Moreover, a negative correlation was found between GSSG and fitness, with a positive correlation with the GSH/GSSG ratio. TG, GSSG, and the GSH/GSSG ratio seem to be reliable markers of oxidative stress in healthy prepubertal children with low fitness or sedentary habits. This research contributes to the recognition that an adequate level of fitness and recreational physical activity in childhood leads to better health and oxidative status.
    No preview · Article · May 2012 · Free Radical Biology and Medicine