Serum Anti‐Carbonic Anhydrase II Antibodies and Oxidant‐Antioxidant Balance in Pre‐eclampsia

Department of Biochemisty, Karadeniz Technical University, Trabzon, Turkey.
American Journal Of Reproductive Immunology (Impact Factor: 2.44). 10/2011; 66(4):297-303. DOI: 10.1111/j.1600-0897.2011.00981.x
Source: PubMed


Citation Aliyazicioglu R, Guven S, Mentese A, Kolayli S, Cengiz S, Deger O, Alver A. Serum anti-carbonic anhydrase II antibodies and oxidant-antioxidant balance in pre-eclampsia. Am J Reprod Immunol 2011; 66: 297–303
Problem The aim of this study was to investigate the presence of anti-carbonic anhydrase II antibodies (anti-CA II) antibodies in pre-eclampsia and the relationships between the autoantibodies, total antioxidant capacity (TAC) and total oxidant capacity (TOC), malondialdehyde (MDA) and oxidative stres index (OSI) parameters.
Method of study We studied 40 early and late onset pre-eclamptic patients and 40 healthy pregnant control and 39 healthy non-pregnant control subjects. Serum CA II antibodies, TAC and TOC, and MDA parameters were studied by ELISA.
Results The mean values for TAC, TOC, OSI, MDA, and anti-CA II were significantly increased in patients with pre-eclampsia compared to the other groups. The anti-CA II antibody levels for the pregnant control subjects were 0.129 ± 0.04 and that for the pre-eclamptic patients were 0.282 ± 0.18. In this study, any absorbance value higher than 0.136, the mean absorbance + 2 S.D. of pregnant control subjects, was defined as positive. Positive results were obtained in 29 of 40 pre-eclamptic patients (72.5%). There were significant positive correlations between serum anti-CA II antibodies and TOC, MDA levels, and OSI levels.
Conclusion The results suggest that anti-CA II antibodies and impairment in oxidant-antioxidant balance may be involved in multifactorial etiology of pre-eclampsia.

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    • "In addition, it is not possible to ascertain that the nuclear translocation of NF-kB is due to TLR-4 signaling or to some other NF-kB activation pathway. Due to the limited number of patients, it is notpossible to divide PE patients in early and late, and it seems that these two conditions have some differences [25] [26], including issues related to the TLR4 pathway [10]. In addition, we measure only IL-6 that clearly did not reflect several aspects of the inflammatory response during preeclampsis development, and future studies must address this lack of information. "
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    ABSTRACT: There was no direct correlation between plasma and placental oxidative damage parameters and inflammation and evidence of TLR4 pathway activation in the placenta in preeclamptic (PE) patients. 33 PE patients and 33 normotensive pregnant women were included. The maternal section of the placenta and blood were collected to the determination of oxidative damage markers (thiobarbituric acid reactive species and protein carbonyls), inflammatory response (interleukin-6 and myeloperoxidase activity), and activation of the TLR-4-NF-kB pathway. An increase of IL-6 levels in both plasma and placenta was observed, but myeloperoxidase activity was not significantly different comparing the groups. Oxidative damage parameters were increased in plasma and placenta in PE patients. A significant increase of the protein levels of TLR-4 and NF-kB was observed in the placenta. The TLR4-NF-kB pathway is upregulated in PE, probably generating local and systemic inflammatory response that is followed by local and systemic oxidative damage.
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    ABSTRACT: Aim: The aim of this study is to investigate anti-carbonic anhydrase antibodies (anti-CA I and CA II antibodies) in the sera of women with polycystic ovary syndrome (PCOS). Methods: In this study serum anti-CA I and II antibody levels of age and BMI matching fifty women with PCOS and fifty women without PCOS on day three of menstrual cycle were assessed with an ELISA method previously developed by Hosoda and modified by Alver et al. Results: The mean serum anti-CA I antibody levels were significantly higher in women with PCOS and anti-CA II antibody levels were not significantly different in women with PCOS compared with control subjects. For serum anti-CA I antibody, the absorbance higher than 0.484 (mean + 3SD of control subjects) was taken as positive, Anti-CA I antibody was detected in 13 of 50 patients with PCOS (26%). Considering serum anti-CA II antibody, the absorbance higher than 0.654 (mean + 3SD of control subjects) was taken as positive. Anti-CA II antibody was detected in 2 of 50 patients with PCOS (4%). All patients with positive anti-CA II antibody also had positive anti-CA I antibody. Autoantibodies specifically reactive to CA I were found to be present at a higher frequency than CA II in the serum of subjects with PCOS in the present study. Conclusion: The results may suggest that autoimmune responses against CA I may be involved in the pathogenesis of PCOS.
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    ABSTRACT: Purpose: To study the existence of anti-carbonic anhydrase antibodies (anti-CA-I&II) in acute anterior uveitis (AAU) patients and to analyze the relationship between the levels of these antibodies and the total antioxidant capacity (TAC), total oxidant capacity (TOC), oxidative stress index (OSI), and malondialdehyde (MDA) level. Methods: Forty-five AAU cases and 43 healthy controls were enrolled in this prospective study. Results: The average anti-CA I and II antibody levels were 0.433 ± 0.306 and 0.358 ± 0.261 IU/mL, respectively, in the AAU group and 0.275 ± 0.147 and 0.268 ± 0.108 IU/mL, respectively, in the control group (p = 0.004 and p = 0.036, respectively). In addition, it was found that the TOC, OSI, and MDA levels in the AAU subjects were statistically significantly higher than those of the control subjects. Conclusions: These results suggest that autoimmune responses against CA I and CA II and an altered serum oxidant-antioxidant balance may be involved in the pathogenesis of AAU.
    No preview · Article · Sep 2013 · Ocular immunology and inflammation
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