Cypermethrin induced alterations in nitrogen metabolism in freshwater fishes. Chemosphere

Biochemistry Laboratory, Department of Zoology, University of Allahabad, Allahabad 211 002, India.
Chemosphere (Impact Factor: 3.34). 04/2011; 83(4):492-501. DOI: 10.1016/j.chemosphere.2010.12.062
Source: PubMed


In the present study, two fresh water fishes namely, Channa punctatus and Clarias batrachus, were exposed to three sub-acute concentrations of synthetic pyrethroid, cypermethrin, for 96 h to evaluate the role of amino acids in fulfilling the immediate energy needs of fishes under pyrethroid induced stress as well as to find out the mechanism of ammonia detoxification. The experiments were designed to estimate the levels of free amino acid, urea, ammonia and the activities of aspartate aminotransferase (AAT), alanine aminotransferase (AlAT), glutamate dehydrogenase (GDH), glutamine synthetase (GS) and arginase in some of the vital organs like brain, gills, liver, kidney and muscle of both fish species. The significant decrease in the levels of amino acids concomitant with remarkable increase in the activities of AAT, AlAT and GDH in these vital tissues of fish species elucidated the amino acid catabolism as one of the main mechanism of meeting out the immediate energy demand of the fishes in condition of cypermethrin exposure. The levels of ammonia were significantly increased at 10% of 96 h LC(50) of cypermethrin in the different organs such as brain, gills, liver, kidney and muscle of both fish species while 15% and 20% concentrations of 96 h LC(50) of cypermehrin registered remarkable decline in both fish species. The differential increment in the activities of GDH, GS and arginase and in the level of urea established three different alternative mechanisms of ammonia detoxification. The results indicated that in C. punctatus, the prevalent mode of nitrogen excretion is in the form of conversion of ammonia into glutamine and glutamate while in C. batrachus, the excessive nitrogen is excreted in the form of urea synthesized from ammonia.

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Available from: Bechan Sharma, Feb 06, 2014
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    • "Significant increase in asparate and alanine amino transaferase along with increased alkaline phosphatase upon cypemethrin exposure has been reported by Loteste et al. (2013) in Prochilodus lineatus fish. Significant alterations in enzymes of nitrogen metabolism in fish such as AST, ALT, glutamate dehydrogenase and gutamine synthetase in two freshwater fire breathing fish Channa striatus and C. batrachus have been reported by Kumar et al. (2011). Saha and Kaviraj (2009) found a significant increase in glucose level along with marked decrease in glycogen level in Heteropneustes fossilis upon cypermethrin exposure. "
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