Comparison of Two Mycoplasma genitalium Real-Time PCR Detection Methodologies

Department of Microbiology & Infectious Diseases, The Royal Women's Hospital, Melbourne, Australia.
Journal of clinical microbiology (Impact Factor: 3.99). 03/2011; 49(3):1140-2. DOI: 10.1128/JCM.02328-10
Source: PubMed


Established in-house quantitative PCR (qPCR) assays to detect the Mycoplasma genitalium adhesion protein (MgPa) and the 16S rRNA gene were found to be comparable for screening purposes, with a kappa value of 0.97
(95% confidence interval [CI], 0.94 to 1.01) and no difference in bacterial load quantified (P = 0.4399).

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    • "One of the major reasons for little studies in this area might be fastidious nature of M genitalium to culture that limited direct detection and required researchers to develop other methods. Nucleic acid amplification-based methods have widely used for detection of M genitalium in urogenital specimens (de Barbeyrac et al., 1993; Jurstrand et al., 2005; Edberg et al., 2008; Ouzounova-Raykova et al., 2011; Twin et al., 2011 "
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    • "This sample size was selected in order to obtain a precision of 4% around a sensitivity of 97%. We found a kappa value of 0.97 (95% CI: 0.94 to 1.01), (sensitivity 95.0% and specificity 99.6%) between the two assays [18]. Women were given a positive MG diagnosis if they tested positive using either assay; this accounted for any possible DNA degradation of the sample during the storage period, and also, in the absence of a clear gold standard for the diagnosis of MG, it was clinically important to treat all women who were MG positive by either assay. "
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