Article

Cdk1 Is Required for the Self-Renewal of Mouse Embryonic Stem Cells

College of Life Sciences, Capital Normal University, Beijing, China.
Journal of Cellular Biochemistry (Impact Factor: 3.26). 03/2011; 112(3). DOI: 10.1002/jcb.23010
Source: PubMed

ABSTRACT

Cyclin-dependent kinase 1 (Cdk1) is indispensible for the early development of the embryo. However, its role in maintaining the undifferentiated state of the embryonic stem (ES) cells remains unknown. In this study, we dissected the function of Cdk1 in mouse ES cells by RNA-interference and gene expression analyses. Cdk1 expression is tightly correlated with the undifferentiated state of the ES cells. Upon differentiation, Cdk1 expression reduced drastically. Cdk1 knock-down by RNA interference resulted in the loss of proliferation and colony formation potential of the ES cells. Consequentially, expression of self-renewal genes was reduced while differentiation markers such as Cdx2 were induced. Our results suggest a role for Cdk1 in maintaining the unique undifferentiated and self-renewing state of the mouse ES cells. © 2010 Wiley-Liss, Inc.

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    • "AURKB has also been associated with TMZ susceptibility[23]and aggressive outcomes of glioblastomas[24]. CDK1 is also known to play regulatory roles in the self-renewal of mouse embryonic stem cells[25]as well as for cell survival of glioblastoma[26]. These findings may support that the selective targeting of these genes for G1 recurrent tumors might be beneficial in the clinic. "
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    • "This finding is consistent with previous observation that Cdk1-depleted ES cells exhibit a dramatic increase in Cdx2 transcript [16]. As shown by our previous study, Cdx2 is one of the highlighted genes whose expression shows significant changed [16]. For example , Mesoendoderm marker Msx1 and ectoderm Fgf5 are also greatly up-regulated upon Cdk1 depletion. "
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    Full-text · Article · Oct 2012 · FEBS letters
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    • "Cyclin-dependent kinase 1 (Cdk1) is indispensable for the early development of the embryos. Cdk1 expression is tightly correlated with the undifferentiated state of ES cells by maintaining the unique undifferentiated and self-renewing state of mouse ES cells [55], whereas Cdk1 has a crucial role in orchestrating a fine balance between cellular proliferation, cell death and DNA repair in hESCs [56]. Recently, it was shown that the transition of ES cell differentiation from the epiblast state into neuroectodermal progenitors specifically depends on the expression and activator functionality of Zfp521 [57]. "
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