Experimental medical mycological research in Latin America - a 2000-2009 overview

Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela.
Revista iberoamericana de micología 12/2010; 28(1):1-25. DOI: 10.1016/j.riam.2010.11.004
Source: PubMed


Se presenta una revisión de lasmásimportantes líneas de investigación en micología médica experimental en América Latina desde el inicio del siglo XXI (búsqueda bibliográfica desde enero de 2000 a diciembre de 2009). Usando las bases de datos PubMed y LILACS, los autores hemos escogido publicaciones en hongos patógenos de importancia clínica que, de acuerdo a nuestra opinión, son las más relevantes por su novedad, interés e impacto internacional, basadas en investigaciones realizadas totalmente en la región latinoamericana o como parte de esfuerzos colaborativos con laboratorios de otras partes del mundo.
De esta forma, discutimos las siguientes áreas: 1) identificación molecular de patógenos fúngicos; 2) epidemiología clínica y molecular de hongos patógenos prevalentes en la región; 3) biología celular; 4) transcriptoma, genoma, taxonomía y filogenia moleculares; 5) inmunología; 6) vacunas; 7) antifúngicos nuevos o experimentales.

Download full-text


Available from: Eva Burger
  • Source
    • "Paracoccidioides is a thermodimorphic fungus and is the etiologic agent of paracoccidioidomycosis (PCM), an important mycosis in Latin America. The infection is caused by the inhalation of the fungal spores, after which the fungus is able to disseminate from the lungs through the lymphatic system or the bloodstream to any organ or system [20]. The initial treatment of PCM depends on the severity of the disease and may last from 2 to 6 months. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The compound oenothein B (OenB), which is isolated from the leaves of Eugenia uniflora, a Brazilian Cerrado plant, interferes with Paracoccidioides yeast cell morphology and inhibits 1,3-beta-D-glucan synthase (PbFKS1) transcript accumulation, which is involved in cell wall synthesis. In this work we examined the gene expression changes in Paracoccidioides yeast cells following OenB treatment in order to investigate the adaptive cellular responses to drug stress. We constructed differential gene expression libraries using Representational Difference Analysis (RDA) of Paracoccidioides yeast cells treated with OenB for 90 and 180 min. Treatment for 90 min resulted in the identification of 463 up-regulated expressed sequences tags (ESTs) and 104 down-regulated ESTs. For the 180 min treatment 301 up-regulated ESTs and 143 down-regulated were identified. Genes involved in the cell wall biosynthesis, such as GLN1, KRE6 and FKS1, were found to be regulated by OenB. Infection experiments in macrophages corroborated the in vitro results. Fluorescence microscopy showed increased levels of chitin in cells treated with OenB. The carbohydrate polymer content of the cell wall of the fungus was also evaluated, and the results corroborated with the transcriptional data. Several other genes, such as those involved in a variety of important cellular processes (i.e., membrane maintenance, stress and virulence) were found to be up-regulated in response to OenB treatment. The exposure of Paracoccidioides to OenB resulted in a complex altered gene expression profile. Some of the changes may represent specific adaptive responses to this compound in this important pathogenic fungus.
    Full-text · Article · Oct 2013 · BMC Microbiology
  • [Show abstract] [Hide abstract]
    ABSTRACT: La caspofungine est une échinocandine prescrite pour le traitement des infections fongiques invasives causées par Candida sp. et Aspergillus sp. Le but de cette étude est d’évaluer le degré de sensibilité à la caspofungine de Candida sp., isolé à partir d’hémocultures dans l’état du Ceará (Brésil) par la méthode de microdilution. Trente-trois souches de Candida sp. ont été sélectionnées pour le test (sept C. albicans, neuf C. tropicalis et 17 C. parapsilosis) ; ces souches sont le plus souvent isolées dans des infections fongiques en Ceará. Les résultats des tests de sensibilité par microdilution peuvent être lus à 24 ou 48 heures après les tests, sans compromettre les interprétations du test. C. parapsilosis montre les CMI les plus élevées comparativement à celles de C. albicans et C. tropicalis pour la caspofungine.
    No preview · Article · Dec 2011 · Journal de Mycologie Médicale/Journal of Medical Mycology
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The pathogenic role of invasive fungal infections (IFIs) has increased during the past two decades in Latin America and worldwide, and the number of patients at risk has risen dramatically. Working habits and leisure activities have also been a focus of attention by public health officials, as endemic mycoses have provoked a number of outbreaks. An extensive search of medical literature from Latin America suggests that the incidence of IFIs from both endemic and opportunistic fungi has increased. The increase in endemic mycoses is probably related to population changes (migration, tourism, and increased population growth), whereas the increase in opportunistic mycoses may be associated with the greater number of people at risk. In both cases, the early and appropriate use of diagnostic procedures has improved diagnosis and outcome.
    Full-text · Article · Mar 2012 · Current Fungal Infection Reports
Show more