Photothermal nanoblade for patterned cell membrane cutting

Department of Electrical Engineering, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Optics Express (Impact Factor: 3.49). 10/2010; 18(22):23153-60. DOI: 10.1364/OE.18.023153
Source: PubMed


We report a photothermal nanoblade that utilizes a metallic nanostructure to harvest short laser pulse energy and convert it into a highly localized and specifically shaped explosive vapor bubble. Rapid bubble expansion and collapse punctures a lightly-contacting cell membrane via high-speed fluidic flows and induced transient shear stress. The membrane cutting pattern is controlled by the metallic nanostructure configuration, laser pulse polarization, and energy. Highly controllable, sub-micron sized circular hole pairs to half moon-like, or cat-door shaped, membrane cuts were realized in glutaraldehyde treated HeLa cells.

Download full-text


Available from: Tara Teslaa
  • Source
    • "Focusing of light is widely used in optical microprobes where a stable and well-confined beam of photons is scanned or directed over a biological sample or photonic structure. Potential applications of such technologies are numerous, including ultra-precise laser surgery [1], nanoscale patterning [2], piercing of a cell [3], and biomedical optical spectroscopy [4]. Typically, these applications require a combination of high spatial resolution and high power transmission. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Focusing of multimodal beams by chains of dielectric microspheres assembled directly inside the cores of hollow waveguides is studied by using numerical ray tracing. The device designs are optimized for laser surgery in contact mode with strongly absorbing tissue. By analyzing a broad range of parameters it is demonstrated that chains formed by three or five spheres with a refractive index of 1.65-1.75 provide a two-fold improvement in spatial resolution over single spheres at the cost of 0.2-0.4 attenuation in peak intensity of the central focused beam. Potential applications include ultra precise laser ablation or coagulation in the eye and brain, cellular surgery, and the coupling of light into photonic nanostructures.
    Full-text · Article · Feb 2011 · Optics Express
  • [Show abstract] [Hide abstract]
    ABSTRACT: This work presents a novel photothermal nanoblade that utilizes metallic nanostructures to light energy from short laser pulses to generate highly localized and shaped explosive cavitation bubbles which enables rapid cut of a lightly contacting cell membrane of a mammalian cell. Photothermal nanoblade can generate micrometer-sized membrane access ports for delivering large size cargo with high efficiency and high cell viability. Biologic and inanimate cargo over 3-orders of magnitude in size including DNA, RNA, 200 nm polystyrene beads, to 2 μm bacteria have also been successfully delivered into multiple mammalian cell types.
    No preview · Article · Jan 2011 · Proceedings of SPIE - The International Society for Optical Engineering
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The effects of periodical focusing of light were studied in chains of sapphire microspheres with 300 μm diameters assembled either on a substrate or inside capillary tubing. Dye-doped fluorescent microspheres were used as multimodal sources of light in experimental studies. Significant reduction of the focused spot sizes was observed for chains of spheres compared to a single sphere case. Numerical ray tracing simulations were performed for similar chains assembled inside hollow waveguides to be used as an optical delivery system with mid-infrared lasers for ultra-precise surgery. The device designs were optimized for contact conditions during laser surgery involving short optical penetration depths of light in tissue. It is shown that chains of spheres with n around 1.65-1.75 provide a two-fold improvement of the spatial resolution over single spheres. Potential applications of these microprobes include ultraprecise laser procedures in the eye and brain or piercing a cell, and coupling of multimodal beams into photonic microstructures.
    Full-text · Article · Feb 2011 · Proceedings of SPIE - The International Society for Optical Engineering
Show more