The role of catecholaminergic neurons in the hypothalamus and medullary visceral zone in response to restraint water-immersion stress in rats

Article · January 2011with6 Reads
DOI: 10.1007/s12576-010-0119-6 · Source: PubMed
The activity of catecholaminergic neurons in the hypothalamus and the medullary visceral zone (MVZ) in rats in response to restraint water-immersion stress (RWIS) was measured by use of dual Fos and tyrosine hydroxylase (TH) immunohistochemistry. In RWIS rats Fos immunoreactive (Fos-IR) nuclei dramatically increased in the paraventricular nucleus (PVN), the supraoptic nucleus (SON), the dorsal motor nucleus of the vagus (DMV), the nucleus of the solitary tract (NTS), the area postrema (AP), and the ventrolateral medulla (VLM). A small number of TH-immunoreactive (TH-IR) and Fos/TH double-labeling neurons in the PVN, and their absence from the SON, were observed in both RWIS and nonstressed rats. More TH-IR neurons were observed in the MVZ of RWIS rats than in nonstressed rats. In RWIS and nonstressed rats, the percentage of Fos-IR nuclei in TH-IR neurons was 38.0 and 14.3% in the DMV, 34.4 and 9.7% in the NTS, 18.6 and 4.5% in the AP, and 45.7 and 18.9% in the VLM, respectively. In conclusion, catecholaminergic neurons in the MVZ are involved in the response to RWIS; although the PVN and SON also participate in the response to RWIS, the mechanism is not via catecholaminergic neurons.
  • ... In our previous studies, we had noted that waterimmersion restraint-related stress in rats activated both vasopressinergic and oxytocinergic neurons in the PVN and caused serious gastric dysfunction. Meanwhile there were AVP and oxytocin (OT) receptors largely expressed in the soma and/or dendrite membranes of the activated neurons in the DMV [10, 11], suggesting the possible involvement of vasopressinergic/oxytocinergic projections from the PVN to the DMV in modulating the gastric functions. The long-descending oxytocinergic projection from the PVN to the DMV has been reported to serve as a link in the main neural control of the gastric function, indicating the important role of OT in autonomic function. ...
  • ... Many studies have confirmed that the MVZ protein, which fulfills a " tracer " role, can be used as a positioning and functional indicator of neuronal activity (Treger et al., 2008; He et al., 2013). Zhao et al. (2011) replicated MODS caused by cerebral hemorrhage in a rat model using different doses (0.5 and 1.0 U) of collagenase and found that the positive expression of MVZ protein increased after cerebral hemorrhage compared with the control group, and the number of positively expressing MVZ cells was significantly higher in the 1.0 U group than in the 0.5 U group. Thus, it can be seen that severe ACVD may cause changes in MVZ protein expression in the central nervous system , and to a certain extent the quantity of protein induced is related to the stimulation intensity suffered. ...
  • ... These results provide strong evidence for a role of apelin in gastroprotection against gastric lesions induced by WIRS. WIRS, a form of emotional stress, has been widely used as an experimental model for the development acute gastric mucosal lesions [24, 25]. This model mimics clinical acute gastric lesions that are observed as a result of major trauma, sepsis or surgery and is commonly used for studying the mechanism of stress-induced gastric damage262728 . ...
August 2011 · PLoS ONE · Impact Factor: 3.23
    Vasopressin (AVP) and oxytocin (OT) are considered to be related to gastric functions and the regulation of stress response. The present study was to study the role of vasopressinergic and oxytocinergic neurons during the restraint water-immersion stress. Ten male Wistar rats were divided into two groups, control and RWIS for 1h. The brain sections were treated with a dual immunohistochemistry... [Show full abstract]
    June 2009 · The Chinese journal of physiology · Impact Factor: 1.16
      Restraint water-immersion stress (RWIS) of rats induces vagally-mediated gastric dysfunction. The present work explored the effects of different durations of RWIS on neuronal activities of the dorsal vagal complex (DVC) and the nucleus ambiguous (NA) in rats. Male Wistar rats were exposed to RWIS for 0, 30, 60, 120, or 180 min. Then, a c-Fos immunoperoxidase technique was utilized to assess... [Show full abstract]
      March 2011 · The Journal of Physiological Sciences · Impact Factor: 1.90
        The present study was to investigate whether there are functional connections between the hypothalamic supraoptic nucleus (SON) and the stomach, which is the case with the paraventricular nucleus. The rats were divided into four groups. Group I: the neuronal discharge was recorded extracellularly in the NTS, DMV or SON before and after cold physiological saline (4°C) was perfused into the... [Show full abstract]
        February 2010 · Zoological Research
          A diagram of motor neuron pool of ventral horn of spinal cord gray matter in toad was first delineated. Different concentrations (1, 0.5, 0.1, 0.01 mol/L) of excitatory amino acid L-Glu or physiological saline (0.65% NaCl) were then microinjected into the motor neuron pool in a urethane-anaesthetized toad. The contraction curve of the gastrocnemius was then recorded by the BL-420 Physiological... [Show full abstract]
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