Svensson, M et al. Accumulation of eosinophils in intestine-draining mesenteric lymph nodes occurs after Trichuris muris infection. Parasite Immunol. 33, 1-11

Faculty of Life Sciences, University of Manchester, Manchester, UK.
Parasite Immunology (Impact Factor: 2.14). 01/2011; 33(1):1-11. DOI: 10.1111/j.1365-3024.2010.01246.x
Source: PubMed


Eosinophils have recently been demonstrated capable of localizing to lymph nodes that drain mucosal surfaces, in particular during T helper 2 (Th2) responses. Resistance of mice to infection with the gastrointestinal nematode Trichuris muris depends critically on mounting of a Th2 response and represents a useful model system to investigate Th2 responses. Following infection of resistant BALB/c mice with T. muris, we observed accumulation of eosinophils in intestine-draining mesenteric lymph nodes (MLNs). The accumulation of MLN eosinophils was initiated during the second week of infection and peaked during worm expulsion. In contrast, we detected a comparably late and modest increase in eosinophil numbers in the MLNs of infected susceptible AKR mice. MLN eosinophils localized preferentially to the medullary region of the lymph node, displayed an activated phenotype and contributed to the interleukin-4 (IL-4) response in the MLN. Despite this, mice genetically deficient in eosinophils efficiently generated IL-4-expressing CD4(+) T cells, produced Th2 cytokines and mediated worm expulsion during primary T. muris infection. Thus, IL-4-expressing eosinophils accumulate in MLNs of T. muris-infected BALB/c mice but are dispensable for worm expulsion and generation of Th2 responses, suggesting a distinct or subtle role of MLN eosinophils in the immune response to T. muris infection.

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    • "The availability of mice genetically deficient in eosinophils added rigour to the early studies. Thus, despite the presence of eosinophils in the MLN correlating with resistance to infection, (Svensson et al. 2011), mice deficient in eosinophils (ΔdblGATA-1 mice) were still able to expel T. muris (Svensson et al. 2011). Macrophages are pleiotropic cells which are capable of converting between many different phenotypes, and therefore have diverse biological functions, including phagocytosis, the release of cytokines, antigen presentation and tissue repair (Noel et al. 2004). "
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    • "Moreover, neutralization of eosinophils with anti-IL-5 antibody also reduced recruitment of these cells to the site of infection [54]. It has been recently show that eosinophils found in the MLNs of resistant mice have an activated phenotype and can produce IL-4 [56]. It is possible, therefore that they could contribute to the development of the resistance-associated Th2 response. "
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