Design of a novel Flow-And-ShooT microbeam

RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA.
Radiation Protection Dosimetry (Impact Factor: 0.91). 12/2010; 143(2-4):344-8. DOI: 10.1093/rpd/ncq476
Source: PubMed


Presented here is a novel microbeam technology—the Flow-And-ShooT (FAST) microbeam—under development at RARAF. In this system,
cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and
tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the
proposed FAST system, RARAF expects to reach a throughput of 100 000 cells per hour, which will allow increasing the throughput
of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent
cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST
microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

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Available from: Guy Garty
    • "Because microbeams use narrow beams of radiation, with micrometer or submicrometer dimensions, microfluidic devices can be valuable tools for these studies at these suitable length scales. Thus, in 2011, Garty et al. used microfluidics to manufacture a novel microbeam using Flow-And-ShooT technology (FAST)[13]. The microfluidic cartridge is used in combination with the permanent magnet microbeam (PMM) in order that cells, tracked by a highspeed digital camera, can flow through a 200 μm wide and 20 μm thick microfluidic channel and intersect with the microbeam radiation , allowing for a high throughput manipulation of irradiated cells. "
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