Article

Design of a novel Flow-And-ShooT microbeam

RARAF, Columbia University, 136 S. Broadway, Irvington, NY 10533, USA.
Radiation Protection Dosimetry (Impact Factor: 0.91). 12/2010; 143(2-4):344-8. DOI: 10.1093/rpd/ncq476
Source: PubMed

ABSTRACT

Presented here is a novel microbeam technology—the Flow-And-ShooT (FAST) microbeam—under development at RARAF. In this system,
cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and
tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the
proposed FAST system, RARAF expects to reach a throughput of 100 000 cells per hour, which will allow increasing the throughput
of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent
cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST
microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput.

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