A novel cold-regulated gene, COR25, of Brassica napus is involved in plant response and tolerance to cold stress

Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, HuaZhong Normal University, Wuhan 430079, China.
Plant Cell Reports (Impact Factor: 3.07). 11/2010; 30(4):463-71. DOI: 10.1007/s00299-010-0952-3
Source: PubMed


Cold stress, which causes dehydration damage to the plant cell, is one of the most common abiotic stresses that adversely affect plant growth and crop productivity. To improve its cold-tolerance, plants often enhance expression of some cold-related genes. In this study, a cold-regulated gene encoding 25 KDa of protein was isolated from Brassica napus cDNA library using a macroarray analysis, and is consequently designated as BnCOR25. RT-PCR analysis demonstrated that BnCOR25 was expressed at high levels in hypocotyls, cotyledons, stems, and flowers, but its mRNA was found at low levels in roots and leaves. Northern blot analysis revealed that BnCOR25 transcripts were significantly induced by cold and osmotic stress treatment. The data also showed that BnCOR25 gene expression is mediated by ABA-dependent pathway. Overexpression of BnCOR25 in yeast (Schizosaccharomyces pombe) significantly enhanced the cell survival probability under cold stress, and overexpression of BnCOR25 in Arabidopsis enhances plant tolerance to cold stress. These results suggested that the BnCOR25 gene may play an important role in conferring freezing/cold tolerance in plants.

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    • "B. napus shows a strong tolerance to low temperatures due to many effective defensive mechanisms (Williams et al. 1988; Rapacz et al. 2002). Progress has been made toward understanding the functions of AP2/ERF TFs in the response of rapeseed to cold stress (Chen et al. 2011; Ge et al. "
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    ABSTRACT: The APETALA2/ethylene response factor (AP2/ERF) transcription factor (TF) superfamily plays an important regulatory role in signal transduction of the plant responses to various stresses including low temperature. Significant progress has been made in understanding the mechanism of cold resistance in Brassica napus, an important oilseed crop. However, comprehensive studies on the induction and activity of these TFs under low temperature have been lacking. In this study, 132 AP2/ERF genes were identified by transcriptome sequencing of rapeseed leaves exposed to 0, 2, 6, 12, and 24 h of low (4 °C) temperature stress. The genes were classified into 4 subfamilies (AP2, DREB, ERF, and RAV) and 13 subgroups, among which the DREB subfamily and ERF subfamily contained 114 genes, no genes were assigned to soloist or DREB A3 subgroups. One hundred and eighteen genes were located on chromosomes A1 to C9. GO functional analysis and promoter sequence analysis revealed that these genes are involved in many molecular pathways that may enhance cold resistance in plants, such as the low-temperature responsiveness, methyl jasmonate, abscisic acid, and ethylene-responsiveness pathways. Their expression patterns revealed dynamic control at different times following initiation of cold stress; the RAV and DREB subfamilies were expressed at the early stage of cold stress, whereas the AP2 subfamily was expressed later. Quantitative PCR analyses of 13 cold-induced AP2/ERF TFs confirmed the accuracy of above results. This study is the first dynamic analysis of the AP2/ERF TFs responsible for cold stress in rapeseed. These findings will serve as a reference for future functional research on transcription in rapeseed.
    Full-text · Article · Jan 2016 · MGG Molecular & General Genetics
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    • " Toyobo , Osaka , Japan ) was applied in the detection system . Real - time PCR reaction was performed using the Real - time PCR Master Mix ( Toyobo ) according to the manual . The Actin gene was used as an internal control to normalize the data . Relative quantity of the target gene expression level was performed using the comparative Ct method ( Chen et al . , 2011 ) . For crude anti - oxidative enzyme extraction , 0 . 3 g of fresh leaves were ground to a fine powder in liquid nitrogen and then mixed with 4 mL sodium phosphate buffer ( 150 mM , pH 7 . 0 ) and treated with pre - cooling at 4 °C . The homogenate was transferred into a 10 - mL centrifuge tube and then centrifuged for 20 min with 13 ,"
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    ABSTRACT: Cold stress is a key factor limiting resource use in bermudagrass (Cynodon dactylon). Under cold stress, bermudagrass growth is severely inhibited and the leaves undergo chlorosis. Therefore, rigorous investigation on the physiological and molecular mechanisms of cold stress in this turf species is urgent. The objective of this study was to investigate the physiological and molecular alteration in wild bermudagrass under cold stress, particularly the changes of transpiration rate, soluble sugar content, enzyme activities, and expression of antioxidant genes. Wild bermudagrass (C. dactylon) was planted in plastic pots (each 10 cm tall and 8 cm in diameter) filled with matrix (brown coal soil:sand 1:1) and treated with 4°C in a growth chamber. The results displayed a dramatic decline in the growth and transpiration rates of the wild bermudagrass under 4°C temperature. Simultaneously, cold severely destabilized the cell membrane as indicated by increased malondialdehyde content and electrolyte leakage value. Superoxide dismutase and peroxidase activities were higher in the cold regime than the control. The expression of antioxidant genes including MnSOD, Cu/ZnSOD, POD, and APX was vividly up-regulated after cold stress. In summary, our results contributed to the understanding of the role of the antioxidant system in bermudagrass’ response to cold. © 2014 by the American Society for Horticultural Science. All rights reserved.
    Full-text · Article · Jan 2015 · Journal of the American Society for Horticultural Science. American Society for Horticultural Science
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    • "BnCOR25 gene from B. napus was weakly expressed in leaves or roots and strong expressed in flowers, stems, hypocotyls and cotyledons using RT-PCR analysis. BnCOR25 gene transcripts were highly accumulated in roots during cold treatment (Chen et al., 2011). "

    Full-text · Article · Jan 2015 · International Journal of Agriculture and Biology
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