Long-term inhibition of HIV-1 replication with RNA interference against cellular cofactors. Antiviral Res

ArticleinAntiviral research 89(1):43-53 · January 2011with10 Reads
Impact Factor: 3.94 · DOI: 10.1016/j.antiviral.2010.11.005 · Source: PubMed

    Abstract

    In this study we tested whether HIV-1 replication could be inhibited by stable RNAi-mediated knockdown of cellular co-factors. Cell lines capable of expressing shRNAs against 30 candidate co-factors implicated at different steps of the viral replication cycle were generated and analyzed for effects on cell viability and inhibition of HIV-1 replication. For half of these candidate co-factors we obtained knockdown cell lines that are less susceptible to virus replication. For three co-factors (ALIX, ATG16 and TRBP) the cell lines were resistant to HIV-1 replication for up to 2 months. With these cells we could test the hypothesis that HIV-1 is not able to escape from RNAi-mediated suppression of cellular co-factors, which was indeed not detected.

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