Bae H , Roberts DP , Lim HS , Strem MD , Park SC , Ryu CM , Melnick RL , Bailey BA. Endophytic Trichoderma Isolates from Tropical Environments Delay Disease Onset and Induce Resistance Against Phytophthora capsici in Hot Pepper Using Multiple Mechanisms. Molecular Plant-Microbe Interactions

U.S. Department of Agriculture, Agricultural Research Service, Beltsville Agricultural Research Center, Beltsville, MD 20705, USA. [corrected]
Molecular Plant-Microbe Interactions (Impact Factor: 3.94). 03/2011; 24(3):336-51. DOI: 10.1094/MPMI-09-10-0221
Source: PubMed


Endophytic Trichoderma isolates collected in tropical environments were evaluated for biocontrol activity against Phytophthora capsici in hot pepper (Capsicum annuum). Six isolates were tested for parasitic and antimicrobial activity against P. capsici and for endophytic and induced resistance capabilities in pepper. Isolates DIS 70a, DIS 219b, and DIS 376f were P. capsici parasites, while DIS 70a, DIS 259j, DIS 320c, and DIS 376f metabolites inhibited P. capsici. All six isolates colonized roots but were inefficient stem colonizers. DIS 259j, DIS 320c, and DIS 376f induced defense-related expressed sequence tags (EST) in 32-day-old peppers. DIS 70a, DIS 259j, and DIS 376f delayed disease development. Initial colonization of roots by DIS 259j or DIS 376f induced EST with potential to impact Trichoderma endophytic colonization and disease development, including multiple lipid transferase protein (LTP)-like family members. The timing and intensity of induction varied between isolates. Expression of CaLTP-N, encoding a LTP-like protein in pepper, in N. benthamiana leaves reduced disease development in response to P. nicotianae inoculation, suggesting LTP are functional components of resistance induced by Trichoderma species. Trichoderma isolates were endophytic on pepper roots in which, depending on the isolate, they delayed disease development by P. capsici and induced strong and divergent defense reactions.

Download full-text


Available from: Choong-Min Ryu
  • Source
    • "Several mechanisms are reported to work in many cases of individual beneficial plantmicrobe interactions [4]. As a consequence of the interaction of these bioagents [9] or plant colonization [10] [11], the proteome and transcriptome of the plants are reported to change. Thus, it is expected that these microorganisms may reprogramme the plant defense responses, resulting in alteration of plant responses to their environment [12]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The beneficial plant-microbe interactions play crucial roles in protection against large number of plant pathogens causing disease. The present study aims to investigate the growth promoting traits induced by beneficial microbes namely Pseudomonas aeruginosa PJHU15, Trichoderma harzianum TNHU27, and Bacillus subtilis BHHU100 treated singly and in combinations under greenhouse and field conditions to control Sclerotinia sclerotiorum. Plants treated with three microbe consortium enhanced plant growth maximally both in the presence and absence of the pathogen. Increase in plant length, total biomass, number of leaves, nodules and secondary roots, total chlorophyll and carotenoid content, and yield were recorded in plants treated with microbial consortia. Also, a decrease in plant mortality was observed in plants treated with microbial consortia in comparison to untreated control plants challenged with S. sclerotiorum. Furthermore, the decrease in disease of all the treatments can be associated with differential improvement of growth induced in pea. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
    Full-text · Article · Mar 2015 · Journal of Basic Microbiology
    • "Remarkably, some Trichoderma spp. have been isolated from aerial plant parts, although even in that case they were afterwards able to colonize roots (Bae et al., 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Botrytis cinerea is a necrotrophic fungal pathogen causing disease in many plant species leading to economically important crop losses. So far, fungicides are widely used to control this pathogen. However, in addition to their detrimental effects on the environment and potential risks for human health, increasing fungicide resistance has been observed in the B. cinerea population. Biological control, implying the application of microbial organisms to reduce disease, has gained importance as an alternative or complementary approach to fungicides. In this respect, the genus Trichoderma constitutes a promising pool of organisms with potential for B. cinerea control. In the first part we review the specific mechanisms involved in the direct interaction between the two fungi, including mycoparasitism, the production of antimicrobial compounds and enzymes (collectively called antagonism) and competition for nutrients and space. In addition, biocontrol has also been observed when Trichoderma was physically separated from the pathogen, thus implying an indirect systemic plant defence response. Therefore, in the second part we describe the consecutive steps leading to induced systemic resistance (ISR), starting with the initial Trichoderma-plant interaction followed by the activation of downstream signal transduction pathways and ultimately the defence response resulting in ISR (ISR-prime phase). Finally, we discuss the ISR-boost phase, representing the effect of the ISR-priming by Trichoderma spp. on plant responses after additional challenge with B. cinerea.
    No preview · Article · Aug 2014 · Molecular Plant Pathology
  • Source
    • "Beneficial effects can occur for at least the first growing season: because the fungus grows and continues to colonize the roots, in turn the plant also shows increased growth. By this colonization and chemical communication, the physiology of the plant is strongly affected via changes in plant gene expression (Samolski et al. 2012; Bae et al. 2011; Shoresh et al. 2010). Thus, the fungus reprogrammes plant gene expression, resulting in an alteration of plant responses to their environment. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Trichoderma harzianum is a naturally occurring filamentous fungus which solubilizes mineral nutrients and inorganic fertilizers, increasing availability and uptake of nutrients to the plant. Rhizoctonia solani is a major problem for seedlings, causing damping-off and in mature plants causing foot and root rot in the tomato crop, reducing nutrient uptake. The aim of this study is to evaluate the effect of Trichoderma harzianum (BHU-51), Trichoderma harzianum (BHU-105) and their consortium Trichoderma harzianum (BHU-51+BHU-105) on management of R. solani and nutrient levels in the plants.The application of Trichoderma as a seed treatment significantly decreased the incidence of damping-off and increased the vigour index of the plants. The maximum reduction in disease incidence was recorded for the consortium (BHU-51+BHU-105) treatments. The mineral content in treated plants was also higher than untreated pathogen-inoculated controls. Field trials also showed that the consortium produced better results in terms of shoot length, chlorophyll content and yield than the control.The application of Trichoderma in consortium form increased mineral nutrient uptake, reduced disease incidence and obtained a greater yield with reduced chemical pesticide loads, benefitting farmers and consumers.
    Full-text · Article · Jul 2014
Show more