Use of a Gluconobacter frateurii Mutant to Prevent Dihydroxyacetone Accumulation during Glyceric Acid Production from Glycerol

Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan.
Bioscience Biotechnology and Biochemistry (Impact Factor: 1.06). 11/2010; 74(11):2330-2. DOI: 10.1271/bbb.100406
Source: PubMed


To prevent dihydroxyacetone (DHA) by-production during glyceric acid (GA) production from glycerol using Gluconobacter frateurii, we used a G. frateurii THD32 mutant, ΔsldA, in which the glycerol dehydrogenase subunit-encoding gene (sldA) was disrupted, but ΔsldA grew much more slowly than the wild type, growth starting after a lag of 3 d under the same culture conditions. The addition of 1% w/v D-sorbitol to the medium improved both the growth and the GA productivity of the mutant, and ΔsldA produced 89.1 g/l GA during 4 d of incubation without DHA accumulation.

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    • "On the other hand, A. tropicalis NBRC16470 produced 101.8 g/L of glyceric acid with 99% of enantiomeric excess (Table 1) [24]. Identification of pathways responsible for the conversion of glycerol in these microorganisms is expected to allow the use of metabolic engineering strategies to reduce byproduct formation and lead to an industrial producer strain [24,51]. "
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    ABSTRACT: The considerable increase in biodiesel production worldwide in the last 5 years resulted in a stoichiometric increased coproduction of crude glycerol. As an excess of crude glycerol has been produced, its value on market was reduced and it is becoming a "waste-stream" instead of a valuable "coproduct". The development of biorefineries, i.e. production of chemicals and power integrated with conversion processes of biomass into biofuels, has been singled out as a way to achieve economically viable production chains, valorize residues and coproducts, and reduce industrial waste disposal. In this sense, several alternatives aimed at the use of crude glycerol to produce fuels and chemicals by microbial fermentation have been evaluated. This review summarizes different strategies employed to produce biofuels and chemicals (1,3-propanediol, 2,3-butanediol, ethanol, n-butanol, organic acids, polyols and others) by microbial fermentation of glycerol. Initially, the industrial use of each chemical is briefly presented; then we systematically summarize and discuss the different strategies to produce each chemical, including selection and genetic engineering of producers, and optimization of process conditions to improve yield and productivity. Finally, the impact of the developments obtained until now are placed in perspective and opportunities and challenges for using crude glycerol to the development of biodiesel-based biorefineries are considered. In conclusion, the microbial fermentation of glycerol represents a remarkable alternative to add value to the biodiesel production chain helping the development of biorefineries, which will allow this biofuel to be more competitive.
    Full-text · Article · Jul 2012 · Biotechnology for Biofuels
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    • "The growth retardation of this strain on glycerol alone was overcome by addition of sorbitol to the medium. A higher glyceric acid concentration of 89.1 g l -1 was reached with the sldA mutant compared to the parental strain (54.7 g l -1 ) as production dihydroxyacetone as byproduct was avoided (Habe et al., 2010b). Glyceric acid production from raw glycerol pretreated with activated charcoal by Gluconobacter sp. "

    Full-text · Chapter · Nov 2011
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