IGF-I and vitamin C promote myogenic differentiation of mouse and human skeletal muscle cells at low temperatures

Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, 309A Building 15, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902, Japan.
Experimental Cell Research (Impact Factor: 3.25). 11/2010; 317(3):356-66. DOI: 10.1016/j.yexcr.2010.11.001
Source: PubMed


In a previous study investigating the effects of low temperature on skeletal muscle differentiation, we demonstrated that C2C12 mouse myoblasts cultured at 30°C do not express myogenin, a myogenic regulatory factor (MRF), or fuse into multinucleated myotubes. At this low temperature, the myoblasts continuously express Id3, a negative regulator of MRFs, and do not upregulate muscle-specific microRNAs. In this study, we examined if insulin-like growth factor-I (IGF-I) and a stable form of vitamin C (L-ascorbic acid phosphate) could alleviate the low temperature-induced inhibition of myogenic differentiation in C2C12 cells. Although the addition of either IGF-I or vitamin C alone could promote myogenin expression in C2C12 cells at 30°C, elongated multinucleated myotubes were not formed unless both IGF-I and vitamin C were continuously administered. In human skeletal muscle cells, low temperature-induced blockage of myogenic differentiation was also ameliorated by exogenous IGF-I and vitamin C. In addition, we demonstrated that satellite cells of IGF-I overexpressing transgenic mice in single-fiber culture expressed myogenin at a higher level than those of wild-type mice at 30°C. This study suggests that body temperature plays an important role in myogenic differentiation of endotherms, but the sensitivity to low temperature could be buffered by certain factors in vivo, such as IGF-I and vitamin C.

18 Reads
  • [Show abstract] [Hide abstract]
    ABSTRACT: For the purpose of determining the higher-order nonlinear optical susceptibility the diffraction method based on investigation into the Fourier-expansion spatial components for the anharmonic grating of the refractive index has been used. Comparison between the experimental and theoretical data makes it possible to relate unambiguously the energy efficiency of multiwave mixing to the nonlinear susceptibility. Efficiency of the proposed mixing patterns has been tested experimentally on the basis of dye solutions in conditions of pulsed laser excitation. The measurement results obtained for the energy efficiency of four-, six- and eight-wave mixing enable calculations of the third, fifth- and seventh-order nonlinear optical susceptibilities, respectively. Experimentally, the method proposed for measurements of the higher order nonlinearities has been realized with the use of the multiwave mixing of monopulse Nd:YAG laser radiation in the solution of polymethine and Rhodamine 6G dyes.
    No preview · Conference Paper · Oct 2005
  • [Show abstract] [Hide abstract]
    ABSTRACT: MicroRNAs (miRNAs) are a recently discovered class of small non-coding RNAs, which are approximately 22 nucleotides in length. miRNAs negatively regulate gene expression by translational repression and target mRNA degradation. It has become clear that miRNAs are involved in many biological processes, including development, differentiation, proliferation, and apoptosis. Interestingly, many miRNAs are expressed in a tissue-specific manner and several miRNAs are specifically expressed in cardiac and skeletal muscles. In this review, we focus on those miRNAs that have been shown to be involved in muscle development. Compelling evidences have demonstrated that muscle miRNAs play an important role in the regulation of muscle proliferation and differentiation processes. However, it appears that miRNAs are not essential for early myogenesis and muscle specification. Importantly, dysregulation of miRNAs has been linked to muscle-related diseases, such as cardiac hypertrophy. A mutation resulting in a gain-of-function miRNA target site in the myostatin gene leads to down regulation of the targeted protein in Texel sheep. miRNAs therefore are a new class of regulators of muscle biology and they might become novel therapeutic targets in muscle-related human diseases.
    No preview · Article · May 2007 · DNA and Cell Biology
  • [Show abstract] [Hide abstract]
    ABSTRACT: In this study, we detected the expression of IGF-I, IGF-II, IGF-IR, IGF-IIR, and IGFBP-3 mRNA at 50 (E50), 70 (E70), and 90 (E90) days of gestation, and 1 (D1), 20 (D20), 70 (D70), 120 (D120), and 180 (D180) days of age in the longissimus dorsi (LD) and the semitendinosus (ST) of pigs from a Yorkshire boar×Erhualian sow (YE) cross as well as a Erhualian boar×Yorkshire sow (EY) cross. We found that the expression of IGF-I and IGF-II mRNA in skeletal muscle tissues differed based on developmental age and reciprocal cross type (P<0.05). The expression of IGF-I mRNA exhibited a fluctuant ascending trend. In contrast, IGF-II showed a fluctuant descending trend after birth. The levels of IGF-IR mRNA were higher before birth compared with after birth except for the ST of EY pigs at D120 (P<0.05). The expression of IGF-IIR and IGFBP-3 mRNA remarkably changed with age and reciprocal cross type (P<0.05). IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D. These data suggest that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues of pigs from reciprocal crosses at different developmental stages and may show linked expression during certain periods of development. Our results may provide a valuable resource for the molecular breeding of pigs.
    No preview · Article · May 2011 · General and Comparative Endocrinology
Show more