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Kong YH, Teather R, Forster R.. Composition, spatial distribution, and diversity of the bacterial communities in the rumen of cows fed different forages. FEMS Microbiol Ecol 74: 612-622

Lethbridge Research Centre, Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
FEMS Microbiology Ecology (Impact Factor: 3.57). 09/2010; 74(3):612-22. DOI: 10.1111/j.1574-6941.2010.00977.x
Source: PubMed
ABSTRACT
The species composition, distribution, and biodiversity of the bacterial communities in the rumen of cows fed alfalfa or triticale were investigated using 16S rRNA gene clone library analyses. The rumen bacterial community was fractionated and analyzed as three separate fractions: populations in the planktonic, loosely attached to rumen digesta particles, and tightly attached to rumen digesta particles. Six hundred and thirteen operational taxonomic units (OTUs) belonging to 32 genera, 19 families, and nine phyla of the domain Bacteria were identified from 1014 sequenced clones. Four hundred and fifty one of the 613 OTUs were identified as new species. These bacterial sequences were distributed differently among the three fractions in the rumen digesta of cows fed alfalfa or triticale. Chao 1 estimation revealed that, in both communities, the populations tightly attached to particulates were more diverse than the planktonic and those loosely attached to particulates. S-Libshuff detected significant differences in the composition between any two fractions in the rumen of cows with the same diet and between the communities fed alfalfa and triticale diets. The species richness estimated for the communities fed alfalfa and triticale is 1027 and 662, respectively. The diversity of the rumen bacterial community examined in this study is greater than previous studies have demonstrated and the differences in the community composition between two high-fiber diets have implications for sample selection for downstream metagenomics applications.

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    • "Sandri et al. (2014) 348Zhou et al., 2015). Diet is the main driver of shifts 355 in rumen bacterial communities and this effect has been extensively studied (Kong et al., 2010; 356 Pitta et al., 2010; de Menezes et al., 2011), however, much less in known about the shifts inKong et al., 2010; Pitta et 363 al., 2010; Peng et al., 2015). There is a diverse array of biological functions associated with 364 bacteria from the phylum Bacteroidetes; the most prevailing function being polysaccharide 365 degradation (Thomas et al., 2011). "
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    • "Target organism Target gene T a (°C) Primer conc. (nM) Reference Protozoa 18S rRNA 55 600 [30] Methanogens mcrA 60 900 [31] Domain bacteria 16S rRNA 50 500 [32] Fibrobacter succinogenes 16S rRNA 58 200 [32] Ruminococcus albus 16S rRNA 55 500 [33] Ruminobacter amylophilus 16S rRNA 60 500 [32] Prevotella bryantii 16S rRNA 61 500 [32] Selenomonas ruminantium 16S rRNA 59 500 [32] Clostridium aminophilum 16S rRNA 56 400 [14, 34] doi:10.1371/journal.pone.0150115.t002where C, FS, P, and ST denote the treatment factors of sample class, forage source, period, and sampling time, respectively. "
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    • "At genus level, Clostridium , Ruminococcus and Butyrivibrio were the most represented Firmicutes. They are potent cellulose degraders as well as have the ability to digest pectin and hence are important component in the rumen dietary fiber utilization [44]. Bacteroidetes and Firmicutes population in the rumen and gut collectively involve in the conversion of organic matter to simpler forms [45, 46]. "
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