Article

Biocatalysis—Key to sustainable industrial chemistry

Sigma-Aldrich, Industriestrasse 25, CH-9470 Buchs, Switzerland.
Current Opinion in Biotechnology (Impact Factor: 7.12). 10/2010; 21(6):713-24. DOI: 10.1016/j.copbio.2010.09.016
Source: PubMed

ABSTRACT

The ongoing trends to process improvements, cost reductions and increasing quality, safety, health and environment requirements of industrial chemical transformations have strengthened the translation of global biocatalysis research work into industrial applications. One focus has been on biocatalytic single-step reactions with one or two substrates, the identification of bottlenecks and molecular as well as engineering approaches to overcome these bottlenecks. Robust industrial procedures have been established along classes of biocatalytic single-step reactions. Multi-step reactions and multi-component reactions (MCRs) enable a bottom-up approach with biocatalytic reactions working together in one compartment and recations hindering each other within different compartments or steps. The understanding of the catalytic functions of known and new enzymes is key for the development of new sustainable chemical transformations.

Download full-text

Full-text

Available from: Roland Wohlgemuth, Dec 31, 2014
  • Source
    • "Nowadays, most of the flavor compounds on the world market are produced by chemical synthesis or by direct extraction from plants (Bicas et al., 2010). However, chemical synthesis results frequently in environmentally unfriendly production processes and the undesirable formation of byproducts , thereby reducing process efficiency and increasing downstream cost (Longo and Sanromán, 2006; Wohlgemuth, 2010). Besides, natural flavor production by extraction from plants has some problems. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Microbial transformation of propenylbenzenes may offer a cleaner and cheaper alternative to natural production of flavors and fragrances. In the present study, the biotransformation of trans-anethole using cells of a Colombian strain of the fungus Colletotrichum acutatum was investigated. Initially, fungitoxicity of this compound against C. acutatum was evaluated; trans-anethole displayed a relatively weak toxicity against the microorganism (<70%, at 200 μg/mL and after 48 hours) and apparently a detoxification mechanism was present. Then, the microorganism was incubated with the substrate at room conditions, using three different culture media (Czapek-Dox, Sabouraud and PDB). Results show that trans-anethole is mainly degraded through an epoxide-diol pathway (trans-anethole to anethole-epoxide, then to syn- and anti-anethole-diol, p-anisaldehyde, p-anisic acid and p-anisic alcohol). However, other minor metabolites [e.g. 3-(4-methoxyphenyl)-1-propanol, 1-(4-methoxyphenyl)-2-propanol, ethyl ester of anisic acid], possibly proceeding from other metabolic pathways were also found. Additionally, it was demonstrated that the concentration of metabolic products is dependent on culture medium used, being anethole-diol the major product obtained in all media used. Interestingly, some of the compounds generated in the biotransformation have been utilized as flavors and fragrances. Based on the identified metabolites, a possible metabolic pathway of the biotransformation of trans-anethole by C. acutatum was proposed.
    Full-text · Article · Dec 2015 · Revista mexicana de ingeniería química
  • Source
    • "In order to meet the demands of the pharmaceutical industry, many companies turned to bioconversions to replace parts of their chemical processes (Kieslich, 1997; Michels & Rosazza, 1999; Rogers, 1999; Wohlgemuth, 2010). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Modern research has focused on the microbial transformation of a huge variety of organic compounds to obtain compounds of therapeutic and/or industrial interest. Microbial transformation is a useful tool for producing new compounds, as a consequence of the variety of reactions for natural products. This article describes the production of many important compounds by biotransformation. Emphasis is placed on reporting the metabolites that may be of special interest to the pharmaceutical and biotechnological industries, as well as the practical aspects of this work in the field of microbial transformations.
    Full-text · Article · Sep 2015 · Critical Reviews in Biotechnology
    • "Biocatalysts are whole microbial cells or enzymes that can be used in biochemical reactions of modern biotechnology. Some of these reactions have optimized or even replaced existing processes (Wohlgemuth, 2010; Resch et al., 2011). Interest in biocatalysts has recently increased with the growth and development of biotechnology as a strategy towards attaining a biobased economy. "
    [Show abstract] [Hide abstract]
    ABSTRACT: In the last decade, attention to extreme environments has increased because of interests to isolate previously unknown extremophilic microorganisms in pure culture and to profile their metabolites. Microorganisms that live in extreme environments produce extremozymes and extremolytes that have the potential to be valuable resources for the development of a bio-based economy through their application to white, red, and grey biotechnologies. Here, we provide an overview of extremophile ecology, and we review the most recent applications of microbial extremophiles and the extremozymes and extremolytes they produce to biotechnology.
    No preview · Article · Aug 2015 · Applied Microbiology and Biotechnology
Show more