Evaluation of disease modifying activity of Colchicum luteum Baker in experimental arthritis

Department of Pharmacology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India.
Journal of ethnopharmacology (Impact Factor: 3). 10/2010; 133(2):303-7. DOI: 10.1016/j.jep.2010.09.027
Source: PubMed


Colchicum luteum (CL) has been traditionally used in the Unani system of medicine as a chief ingredient of many polyherbal formulations for the treatment of joint pain and rheumatoid arthritis (RA).
To evaluate the antiarthritic activity of CL hydroalcoholic extract (CLHE) in formaldehyde and complete Freund's adjuvant (CFA) induced arthritis.
Arthritis was induced by administration of either formaldehyde (2% v/v) or CFA into the subplantar surface of the hind paw of the animal. Joint swelling was measured on days 8, 9 and 10 in formaldehyde induced arthritis and days 3, 7, 14 and 21 in CFA induced arthritis. In order to evaluate the effect of CLHE on disease progression, serum TNF-α level and synovial expression of proinflammatory mediators (TNF-R1, IL-6 and IL-1β) was determined in CFA induced arthritis.
CLHE produced a significant and dose dependent inhibition of joint swelling during the entire duration of the study in both, formaldehyde and CFA induced arthritis. Serum TNF-α level was also reduced significantly in a dose dependent manner in all the CLHE treated groups. The expression of proinflammatory mediators (TNF-R1, IL-6 and IL-1β) was also found to be less in the CLHE treated group as compared to control.
We believe that the antiarthritic activity of CLHE was due to its modulatory effect on the expression of proinflammatory cytokine in the synovium. Our results contribute towards validation of the traditional use of CL in the treatment of RA and other inflammatory joint disorders.

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    • "Dosage selection for fractions of plant (ethyl acetate, 100 mg/kg and n-butanol, 200 mg/kg) was based on the previous studies conducted by our research group [20]. The antiarthritic potential of EAPA and BPA was initially investigated against formaldehyde- induced paw edema, which is one of the most common methods for screening of antiarthritic agents [37]. The localized inflammation, induced by injection of formaldehyde into rat paw, is biphasic in nature [38]. "
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    Full-text · Article · Jun 2014 · BioMed Research International
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    • "Arthritis is an autoimmune disease in which there is joint inflammation, synovial proliferation and destruction of articular cartilage (Tripathi 2003). It is a common disease having peak incidence in 3 rd to 4 th decades of life with 3-5 times higher preponderance in female (Mohan 2000). Its prevalence depends upon age (Pandey 2010). "
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    Full-text · Dataset · Nov 2013
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    • "Researchers have found many new mediators viz, TNF-毩, IL-1 and enzymes which take part directly or indirectly in perpetuation of RA[12] [13] [14]. Previous reports indicated that hydroalcoholic extract of root of Hemidesmus indicus R.Br. "
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