Article

Purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures

BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar 388 120, Anand, Gujarat, India.
Bioresource Technology (Impact Factor: 4.49). 09/2010; 102(2):1795-802. DOI: 10.1016/j.biortech.2010.09.025
Source: PubMed

ABSTRACT

The present study is focused on purification, characterization and comparison of phycoerythrins from three different marine cyanobacterial cultures--hormidium sp. A27 DM, Lyngbya sp. A09 DM and Halomicronema sp. A32 DM. 'Phycoerythrin' was successfully purified and characterized. On SDS-PAGE, the PE purified from all three young cultures showed four bands--corresponding to α and β subunits of each of PE-I and PE-II. However, phycoerythrin purified after prolonged growth of Phormidium sp. A27 DM and Halomicronema sp. A32DM showed only one band corresponding to 14 kDa whereas Lyngbya sp. A09 DM continued to produce uncleaved phycoerythrin. The absorption spectra of purified PEs from all the three young and old cultures showed variations however the fluorescence studies of the purified PEs in all cases gave the emission spectra at around 580 nm. The described work is of great importance to understand the role of phycoerythrin in adapting cyanobacteria to stress conditions.

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    • "Each subunit heterodimer has one to four (rings A, B, C and D) linear tetrapyrrole chromophores which are covalently attached to cysteines in the apoproteins with a thioether bond at the C-3 position on ring A and in some cases by an additional thioether bond at the C-18 position on ring D[3]. The pathway of energy transfer within the subunits of PBPs starts from PE to PC to APC and finally reaches Chl a for photosynthesis[4,5]. Cyanobacteria can synthesize PBPs up to 50 % of its total soluble protein[6]. "
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    • "A32DM, Pseudanabaena tenuis , Spirulina fusiformis , Arthronema africanum , Calothrix sp., Oscillatoria quadripunctulata , Pseudanabaena sp. Minkova et al. ( 2007 ), Santiago- Santos et al. ( 2004 ), Soni et al. ( 2010 ), Minkova et al. ( 2007 ), Mishra et al. ( 2008 ), Su et al. ( 2010 ), Cano-Europa et al. ( 2010 ), Parmar et al. ( 2011 ) and Mishra et al. ( 2011 ) Sonication Phycoerythrin Cyanosarcina sp. SK40, Phormidium sp. "
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    • "The filamentous cyanobacterium Phormidium rubidum A09DM (formally known as Lyngbya sp. A09DM) was isolated from sea shores of Okha, Gujarat, India, and identified by 16S rRNA gene sequence analysis (accession No. HM446280) (Parmar et al., 2011). The morphology of the alga was observed by using a light (BX41TF, Olympus Corporation, Tokyo, Japan) and scanning electron microscopy (Philips XL 30 ESEM). "
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    ABSTRACT: The functionality and stability of phycobiliproteins (PBPs) phycoerythrin (PE), phycocyanin (PC) and allophycocyanin (APC) were investigated under various temperatures, pHs and oxidative stressors. All PBPs were thermostable up to 4-40°C; however, their concentration decreased rapidly at 60-80°C. The maximum stability of all PBPs was in the pH range 6.0-7.0. Decrease in PBPs content was found under high acidic (pH 2-4) and alkaline conditions (pH 8-12). The oxidizing agent (0.1-0.6%) showed the least effect on the stability of PBPs; however, 0.8-1.0% H2O2 caused significant loss of PBPs. Contrary to PE, PC and APC was more susceptible to an oxidizing agent. The chromophore associated with α- and β-subunit of PBPs and thus, their functionality (fluorescence) was severely affected under high temperature (60-80°C), and oxidizing agent, as well as low (2-4) and high (8-12) pH. Contrary to PC and APC, functionality of PE was surprisingly maintained even at pHs 6-12 and under oxidative stress. Copyright © 2015 Elsevier Ltd. All rights reserved.
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