No effect of melatonin on oxidative stress after laparoscopic cholecystectomy: A randomized placebo-controlled trial

Department of Surgical Gastroenterology, University of Copenhagen, Herlev Hospital, Herlev, Denmark.
Acta Anaesthesiologica Scandinavica (Impact Factor: 2.32). 10/2010; 54(9):1121-7. DOI: 10.1111/j.1399-6576.2010.02294.x
Source: PubMed


Melatonin, an endogenous circadian regulator, also has antioxidant and anti-inflammatory properties. The aim of this study was to evaluate the antioxidative effect of melatonin in patients undergoing laparoscopic cholecystectomy.
Patients were randomized to receive 10 mg melatonin or placebo during surgery. Blood samples for analysis of malondialdehyde (MDA), ascorbic acid (AA), total ascorbic acid (TAA) dehydroascorbic acid (DHA) and C-reactive protein (CRP) were collected pre-operatively and at 5 min, 6 h and 24 h after operation.
Twenty patients received melatonin and 21 patients received placebo during surgery. No significant differences were observed between the groups in the oxidative stress variables MDA, TAA, AA and DHA or in the inflammatory variable CRP (repeated-measures ANOVA, P>0.05 for all variables).
Administration of 10 mg melatonin did not reduce variables of oxidative stress in patients undergoing elective laparoscopic cholecystectomy.

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    • "AA, DHA and TAA were stabilized with 10% meta-phosphoric acid containing 2 mM disodium EDTA before storage. Both MDA and AA were analysed using a high pressure liquid chromatograph method as described previously [18], [19]. "
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