Song, JM, Kim, YC, Barlow, PG, Hossain, MJ, Park, KM, Donis, RO et al.. Improved protection against avian influenza H5N1 virus by a single vaccination with virus-like particles in skin using microneedles. Antiviral Res 88: 244-247

Department of Microbiology and Immunology, Emory University School of Medicine, Atlanta, GA, USA.
Antiviral research (Impact Factor: 3.94). 11/2010; 88(2):244-7. DOI: 10.1016/j.antiviral.2010.09.001
Source: PubMed


To develop a more effective vaccination method against H5N1 virus, we investigated the immunogenicity and protective efficacy after skin vaccination using microneedles coated with influenza virus-like particles containing hemagglutinin derived from A/Vietnam/1203/04 H5N1 virus (H5 VLPs). A single microneedle vaccination of mice with H5 VLPs induced increased levels of antibodies and provided complete protection against lethal challenge without apparent disease symptoms. In contrast, intramuscular injection with the same vaccine dose showed low levels of antibodies and provided only partial protection accompanied by severe body weight loss. Post-challenge analysis suggested that improved protection was associated with lower lung viral titers and enhanced generation of recall antibody secreting cells by microneedle vaccination. Thus, this study provides evidence that skin delivery of H5 VLP vaccines using microneedles designed for self-administration induces improved protection compared to conventional intramuscular immunization.

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    • "In addition, the administration of VLP vaccines via microneedles was shown to induce superior levels of recall immune responses compared to conventional intramuscular immunization [29]. Influenza VLPs expressing the HA subunit were coated on solid metal microneedles and manually applied onto the skin of mice, which induced comparable antibody responses to intramuscular administration, and full protection against viral challenge [30, 31]. Moreover, another study compared the immune responses elicited by low-dose microneedle and low-dose intramuscular routes, which reported that the low-dose microneedle induced higher immune responses that were similar to the serological antibody titers produced by high-dose intramuscular route [32]. "
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