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BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (3): 260-264
&
Abstract
is study was aimed to depict potential eff ects of stable formulation (water in oil emulsion),
containing green tea (Camellia sinensis L) extract on skin sebum production in healthy hu-
man volunteers.
For this purpose formulation was designed using ethanolic green tea extract and
Abil®EM was used as an emulsifi er. Formulation was applied to the cheeks of healthy hu-
man volunteers (n=) for a period of weeks. Measurements for skin sebum production were
considered using Sebumeter MPA . Results were compiled and any eff ect produced by the
formulation was justifi ed statistically. It was observable that statistically signifi cant (p < . )
results were found for skin sebum production after long term application of the formulation.
formulation of green tea extract was ideal in all aspects and can be experienced in skin dis-
orders like acne to further investigate its eff ects in unhealthy volunteers.
KEY WORDS: Camellia sinensis L, ANOVA, skin sebum, Abil®EM, formulation.
OUTCOMES OF 3%
GREEN TEA EMULSION
ON SKIN SEBUM
PRODUCTION IN
MALE VOLUNTEERS
Tariq Mahmood*
1
, Naveed Akhtar
1
, Barkat Ali Khan
1
,
Haji M Shoaib Khan
1
, Tariq Saeed
2
Department of Pharmacy, Faculty of Pharmacy and Alternative Medicine,
e Islamia University of Bahawalpur, (zip code: ), Bahawalpur, Pakistan
University College of Pharmacy, e University of Punjab,
(zip code: ), Lahore, Pakistan.
* Corresponding author
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (3): 261-264
TARIQ MAHMOOD ET AL.: OUTCOMES OF 3% GREEN TEA EMULSION ON SKIN SEBUM PRODUCTION IN MALE VOLUNTEERS
Introduction
Skin is considered excellent model for non-invasive
topical delivery. Topical emulsions have widespread
acceptability by the user and have been used as der-
matological formulations since long time (). Water
in oil type emulsions are frequently used because of
their ease of application, restriction of water evapora-
tion from skin surface and by their emollient action ().
More than years ago in Chinese history, em-
peror Sin-Non described that a daily cup of tea could
be handy to dissolve number of poisons in our body.
It was strongly believed in ancient culture that tea
can prevent and treat many diseases. But scientifi cally
these benefi cial eff ects are described more recently ().
Tea (Camellia sinensis L) of eaceae has many kinds
but three well known types are: green (un-fermented),
oolong (semi-fermented), and black (fully-fermented).
By inactivating the enzymes in the fresh leaves green
tea is made either by fi ring or by steaming, thus to pre-
vent the enzymatic oxidation of catechins (). Catechins
(polyphenols) are main active constituents found in tea
(). ere are several polyphenolic catechins in green
tea, viz. (−) epicatechin (EC), (−) epicatechingallate
(ECG), (−) epigallocatechin(EGC), (−) epigallocate-
chin--gallate (EGCG), (+)catechin, and (+) gallocat-
echin (GC) Figure . EGCG is the most found catechin
in green tea () of the total catechin contents ().
Tea preparations have shown to trapping activity against
various reactive oxygen species (ROS) such as singlet
oxygen, hydroxyl radical, superoxide radical, nitric ox-
ide, peroxynitrite and nitrogen dioxide and were helpful
in reducing damage to proteins, lipid membranes and
nucleic acid in cell-free systems (). Furthermore epi-
demiological studies suggest that tea is eff ective against
variety of cancers and well known chemo preventive
agent. Several other epidemiological studies suggest
green tea eff ective in oral diseases, bone disorders, neu-
rodegenerative and cardiovascular disorders (). The
problem with green tea polyphenols was their strong
affi nities for many proteins like milk, casein and their
interference with digestive enzymes that results in re-
duced lipid, starch and proteins digestibility. ey have
shown to interfere with zinc, iron and sodium as well ().
Moreover catechins are subject to extensive biotrans-
formation including methylation, glucuronidation, sul-
fation and ring fi ssion metabolism. e potential health
eff ects of catechins depend on the amount consumed
and on their bioavailability. The plasma concentra-
tions of EGCG were much lower when given orally ().
Aim of this study was to test this formulation in healthy
human volunteers for its eff ects on skin sebum produc-
tion as many of the active constituents in green tea are
prompted to contain anti-sebum effects declared by
a study that green tea decreases hormonal activity be-
cause tea galltes and α- linoleic acid are selective inhibi-
tors of α-reductase (). Furthermore green tea reduc-
es infl ammation due to lipids present in green tea such
as Linoleic acid and α- linoleic acid () which reduces
microcomedone size in micrcomedonal acne (). It has
antimicrobial activity (), P. acnes was most sensitive
with green tea fl avor compounds (). Green tea is also
powerful antioxidant (). erefore keeping in mind
all above points of view, a desired dermatological for-
mulation was of esteemed value using green tea extract.
Material and Methods
Detailed procedure is previously reported in ()
and briefly summarized here. Camellia sinensis
leaves extract was prepared by maceration. For this
purpose grams powdered Camellia sinensis
leaves were drenched with analytical grade etha-
nol for days. Filtered extract was evaporated at
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (3): 262-264
TARIQ MAHMOOD ET AL.: OUTCOMES OF 3% GREEN TEA EMULSION ON SKIN SEBUM PRODUCTION IN MALE VOLUNTEERS
o
C under vacuum, using a rotary evaporator. The
blackish green colored extract was stored at
o
C.
For a formulation paraffin oil ( ) and surfactant
Abil®EM ( ) was heated up to
C±
C. At the
same time, aqueous phase consisting of water (quantity
suffi cient to make ) was heated to
C±
C and
then the green tea extract ( ) was added in this aque-
ous phase. After that, aqueous phase was added to the oil
phase drop by drop and stirred with homogenizer to ob-
tain a formulation with dependable consistency. Mean
while few drops of lemon oil were added during this
stirring time to give good fragrance to the formulation.
Study design
For application of formulation, volunteers were cho-
sen whose ages were in between and years. Only
male volunteers were included in this work. Volunteers
were examined for any serious skin disease or damage
especially on cheeks and forearms. Each volunteer was
provided with a volunteer protocol before the study.
is protocol stating each volunteer signed the terms
and conditions of the testing individually. Volunteers
were not informed about the contents of the formula-
tions. Skin tests were performed at
o
C and relative
humidity conditions. Before application of formulation a
patch test was performed on forearms of the volunteers
for hours to check any irritation in the formulation.
Each volunteer on the second day was provided with
formulation and volunteers were instructed properly
about the application of formulation. Each individual
was instructed to come for measurements of readings
for skin sebum production on week , , , , , and .
Mathematical and Statistical Analysis
The percentage changes for the individual values
of different parameters, taken every week, of vol-
unteers were calculated by the following formula;
Percentage Change = ((A – B) / B)*
Here;
A = Individual value of any parameter (from
st
to
th
week)
B = Zero hour value of that parameter
The measured values obtained for skin sebum pro-
duction were analyzed using SPSS . on the PC
computer (ANOVA for variation between differ-
ent time intervals and level of signifi cance was , ).
Results and Discussion
In-vitro evaluation of creams
Stability of the formulation was evaluated using dif-
ferent conditions of storage i.e. ˚C, ˚C and ˚C +
RH (relative humidity). In this study no liquefac-
tion and phase separation were observed in the for-
mulation samples throughout the study period of
days even at elevated temperatures. Abil®EM is a
lipophilic surfactant () and it has been found that
lipophilic surfactants are more stable at elevated tem-
peratures (). Furthermore pH of the formulation was
., considered as normal skin pH range i.e. to ,
(). Detailed description is previously reported in ().
In-vivo characterization of the formulation for skin anti-
sebum eff ects
Sebum is produced by halocrine sebaceous glands
connected to the hair follicles. Sebaceous glands are
found everywhere in the body except in palm and
soles. Sebum acts as lubricant and imparts waterproof-
ing properties to the stratum corneum. Any excessive
sebum secretion is associated with pore enlargement
leading to undesireable pathophysiological condition
called acne (). Topical application of α- linoleic acid
and EGCG ((-)-eppigallocatechin--gallate) in animal
model have shown to inhibit sebum production. is
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (3): 263-264
TARIQ MAHMOOD ET AL.: OUTCOMES OF 3% GREEN TEA EMULSION ON SKIN SEBUM PRODUCTION IN MALE VOLUNTEERS
Conclusion
Above mentioned results for formulation seems to promote the concept about green tea multi factorial benefi ts against
sebum production i.e. tea galltes and α- linoleic acid are selective inhibitors of α-reductase (), α- linoleic acid reduces
microcomedone size in micrcomedonal acne (). Further propagation of this study in unhealthy volunteers might be
foundation to speculate about the functionality of green tea extracts delivered topically in opposition to sebum produc-
tion. Hence we suggest that cheaper green tea emulsion should be tested in conditions like acne vulgaris against any
standard treatment to compare its results in unhealthy states.
Acknowledgements
Financial support was given by e Department of Pharmacy, e Islamia University of Bahawalpur, Pakistan. e au-
thors thank to the Chairman, Department of Pharmacy, e Islamia University of Bahawalpur for his support to accom-
plish this task.
Conflict-of-interest policy
Authors do not have any commercial affi liations, or potential confl icts of interest associated with this work submitted
for publication.
Ethical Standards
In this study, the clinical research using human subjects was conducted in accordance with the ethical considerations for
human subjects approved by Board of Advance Studies and Research. Healthy human volunteers (male) were selected
whose ages were in between and years. Prior to the tests, the volunteers were examined by a cosmetic expert for
any serious skin disease or damage especially on cheeks and forearms. Before the study, every volunteer was provided
with a volunteer protocol. is protocol stating the terms and conditions of the testing were signed by every volunteer
individually. Volunteers were not informed about the contents of formulations. All the skin tests were done at
o
C and
relative humidity conditions. On the fi rst day, patch test (i.e. skin sensitivity test) was performed on the forearms of
each volunteer to determine any possible reactions to the topical emulsion.
activity was due to selective inhibition of α-reductase
(), an enzyme found in sebaceous glands (). ere
are two isozymes for α-reductase, type and type .
As α- linoleic acid inhibits both type and type while
EGCG inhibits type at concentration of mmol/l ().
In this study effects of the formulation on skin se-
bum production were noted in volunteers and
results for average percent changes in skin se-
bum production are presented in Figure. .
It was found that formulation decreased the sebum con-
tents throughout the study period of days. When jus-
tifi ed statistically results of ANOVA were signifi cant (p
< .) for the formulation with respect to time. Up to our
knowledge this is fi rst study of its kind in which green
tea extract was tested for anti-sebum secretion eff ects
in male healthy volunteers. Further propagation of this
study in unhealthy states would be more imperative to
state the actual potential of green tea emulsion. Fur-
ther study could be based on the study design by Shar-
quie et al would be sound enough to test this emulsion
in unhealthy states like acne vulgaris. In this study they
have compared tea lotion with zinc sulfate solu-
tion in the treatment of acne. Between the two groups
studied in this study, group treated with tea lotion
was found more eff ective in decreasing number of in-
fl ammatory lesions than zinc sulfate solution. It was
concluded from this study that tea lotion was more
superior in its eff ects and could be superior choice than
zinc sulfate solution in treatment of acne vulgaris ().
We also suggest that cheaper green tea emul-
sion without any added substances could be tested
in conditions like acne vulgaris against any standard
treatment to compare its results in unhealthy states.
BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 2010; 10 (3): 264-264
TARIQ MAHMOOD ET AL.: OUTCOMES OF 3% GREEN TEA EMULSION ON SKIN SEBUM PRODUCTION IN MALE VOLUNTEERS
Abbreviations
ROS - Reactive Oxygen Species
EGCG - epigallocatechin--gallate
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