Multinucleated giant cells with an osteoclast phenotype derived from caprine peripheral blood mononuclear cells

Università degli Studi di Milano, Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, via Celoria 10, 20133 Milano, Italy.
The Veterinary Journal (Impact Factor: 1.76). 09/2011; 189(3):361-3. DOI: 10.1016/j.tvjl.2010.07.016
Source: PubMed


Formation of multinucleated giant cells (MGCs) by macrophage fusion is a typical cytopathic effect of lentiviral replication in caprine monocytes and MGC formation from cultured caprine peripheral blood mononuclear cells (PBMCs) has been considered to be diagnostic for small ruminant lentivirus (SRLV) infection. In this study, formation of MGCs was observed after 7-14 days when PBMCs were cultured from healthy goats free from SRLV infection. These MGCs expressed tartrate-resistant acid phosphatase, calcitonin receptor, integrin αVβ3, cathepsin K and matrix metalloproteinase 9 and were able to resorb bone in vitro in the absence of RANKL and macrophage colony stimulating factor, consistent with an osteoclast phenotype.

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    • "Viral growth curves analysis was carried out to compare three viral strains (CAEV-TO1/89, Roccaverano and Seui). Since Roccaverano strain did not show CPE in any of the selected fibroblastic cell types and syncitium formation in BDM cultures is not necessarily related to SRLV infection (Pisoni et al., 2010), viral production was measured for both B1 and E prototypes by RT activity. Based on standard curves of each strain, the detection limit of RT activity assay was 10 2 TCID 50 /ml. "
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