Neutral and acidic oligosaccharides in Holstein-Friesian colostrum during the first 3 days of lactation measured by high performance liquid chromatography on a microfluidic chip and time-of-flight mass spectrometry

Article (PDF Available)inJournal of Dairy Science 93(9):3940-9 · September 2010with150 Reads
DOI: 10.3168/jds.2010-3156 · Source: PubMed
Abstract
Oligosaccharides (OS) from bovine milk are a class of bioactive molecules that are receiving increasing commercial attention for their potential health benefits. In the present work we measured, comprehensively and systematically, free milk OS in the colostrum of 7 Holstein-Friesian cows during the first 3 d of lactation in 12-h intervals by HPLC-chip/time-of-flight mass spectrometry to determine the biological variation of free milk OS in early lactation. The high sensitivity and resolution of the analytical technique made it possible to monitor all OS species, thus providing a comprehensive and quantitative analysis of OS variations during colostrum production. This study confirmed that although sialyllactose is the major OS in bovine colostrum, several neutral OS species are present in significant abundance even at the third day of lactation. Furthermore, variation in terms of OS species and relative abundances of OS between cows suggest individual animal variation. These variations are likely due to genetic factors because environmental factors such as nutrition, lactation number, and accommodation were the same for all cows. This investigation revealed that colostrum milk from Holstein-Friesian cows is a rich source of neutral and acidic OS for the food and pharmaceutical industries.
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    • "In a preliminary study, we observed the effect of genotype at s1 -casein locus and the undernutrition on SOS in goat milk (Di Trana et al., 2014 ). On the other hand, milk OS composition is subject to genetic variations in terms of breed, and the number and concentration of glycans decrease rapidly over the course of cow lactation (McJarrow and Van Amelsfort-Schoonbeek, 2004; Barile et al., 2010; Sundekilde et al., 2012) and goat lactation (Claps et al., 2014). So far, few studies have been done on SOS from different goat breeds. "
    [Show abstract] [Hide abstract] ABSTRACT: The fraction of oligosaccharides (OS) in goat milk is becoming increasingly important for its bio-functional role. OS profile of goat milk shows more similarity to human milk in comparison to bovine and ovine milk, in addition goat milk contains sialylated OS which play an important role in brain development and increase immunity in infants. In the first study, in order to improve the knowledge on breed effect on sialyloligosaccharides (SOS), we have focused on three of the most representative SOS; 6' sialyllactose (6'-SL), 3' sialyllactose (3'-SL) and disialyllactose (DSL) in colostrum and milk from Saanen goats, a cosmopolitan breed, and from Garganica goats, native breed. Successively, we compared the SOS levels in colostrum and milk of Saanen goats in two different parity. The results showed a significant effect of breed on SOS content (P < 0.05) and a significant interaction breed x sampling time occurred for 3'-SL (P < 0.001) and 6'-SL (P < 0.01). Concerning parity, a significant effect was found only for the 3'-SL content (P < 0.05). The present finding is particularly relevant in order to preserve and improve native breed production in view of a sustainable land use.
    Full-text · Article · Nov 2015
    • "In recent years, the use of nano-liquid chip-based technologies mainly coupled to MS or tandem MS (MS/MS) techniques have demonstrated to be extremely helpful for OS identification and it has been applied to milk characterization due to its high sensitivity and capacity for compositional verification [4]. Nano-LC-Chip technology coupled to time of flight (TOF) MS has been successfully used for OS analysis of human milk [24], porcine milk [25] and bovine milk [26,27]. An exhaustive characterization of OS in goat's milks with and without the genetic ability to synthesize s1 -casein by nanoflow liquid chromatography–quadrupole-TOF MS (Nano-LC-Chip–Q-TOF MS) with a porous graphitized carbon column has been recently reported [4]. "
    [Show abstract] [Hide abstract] ABSTRACT: A detailed qualitative and quantitative characterization of goat colostrum oligosaccharides (GCO) has been carried out for the first time. Defatted and deproteinized colostrum samples, previously treated by size exclusion chromatography (SEC) to remove lactose, were analyzed by nanoflow liquid chromatography-quadrupole-time of flight mass spectrometry (Nano-LC-Chip-Q-TOF MS). Up to 78 oligosaccharides containing hexose, hexosamine, fucose, N-acetylneuraminic acid or N-glycolylneuraminic acid monomeric units were identified in the samples, some of them detected for the first time in goat colostra. As a second step, a hydrophilic interaction liquid chromatography coupled to mass spectrometry (HILIC-MS) methodology was developed for the separation and quantitation of the main GCO, both acidic and neutral carbohydrates. Among other experimental chromatographic conditions, mobile phase additives and column temperature were evaluated in terms of retention time, resolution, peak width and symmetry of target carbohydrates. Narrow peaks (wh: 0.2-0.6min) and good symmetry (As: 0.8-1.4) were obtained for GCO using an acetonitrile:water gradient with 0.1% ammonium hydroxide at 40°C. These conditions were selected to quantify the main oligosaccharides in goat colostrum samples. Values ranging from 140 to 315mgL(-1) for neutral oligosaccharides and from 83 to 251mgL(-1) for acidic oligosaccharides were found. The combination of both techniques resulted to be useful to achieve a comprehensive characterization of GCO.
    Full-text · Article · Oct 2015
    • "A comprehensive review by Urashima et al. [132] shows that approximately 25 bovine milk OS (BMO) structures had been characterized before 2011. The development of advanced analytic techniques, such as several mass spectrometric methods and hydrophilic interaction liquid chromatography-high performance liquid chromatography, has enabled significant improvement in the identification of new BMO; as many as 40 BMO have been characterized [133, 134]. The low concentration of BMO makes it challenging to identify and characterize these compounds when compared with HMO. "
    [Show abstract] [Hide abstract] ABSTRACT: Milk is the most complete food for mammals, as it supplies all the energy and nutrients needed for the proper growth and development of the neonate. Milk is a source of many bioactive components, which not only help meeting the nutritional requirements of the consumers, but also play a relevant role in preventing various disorders. Milk-derived proteins and peptides have the potential to act as coadjuvants in conventional therapies, addressing cardiovascular diseases, metabolic disorders, intestinal health, and chemopreventive properties. In addition to being a source of proteins and peptides, milk contains complex oligosaccharides that possess important functions related to the newborn's development and health. Some of the health benefits attributed to milk oligosaccharides include prebiotic probifidogenic effects, antiadherence of pathogenic bacteria, and immunomodulation. This review focuses on recent findings demonstrating the biological activities of milk peptides, proteins, and oligosaccharides towards the prevention of diseases of the 21st century. Processing challenges hindering large-scale production and commercialization of those bioactive compounds have been also addressed.
    Full-text · Article · Mar 2015
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