Vol. 22, No. 3, 2010
Received February 2, 2010, Revised March 8, 2010, Accepted for
publication March 8, 2010
*This study was supported by a grant from the SKINMED
CORPORATION. This study was also supported by a grant from the
National Research Foundation of Korea (KRF-2008-314-E00152).
Corresponding author: Jeung-Hoon Lee, M.D., Department of Derma-
tology, School of Medicine, Chungnam National University, 33
Munhwa-ro, Daejeon 301-040, Korea. Tel: 82-42-280-7707, Fax:
82-42-280-8459, E-mail: firstname.lastname@example.org
Ann Dermatol Vol. 22, No. 3, 2010DOI: 10.5021/ad.2010.22.3.255
Enhancement of Keratinocyte Differentiation by Rose
Jin-Hwa Kim, Ph.D., Dae-Kyoung Choi, Ph.D.1, Sang-Sin Lee, M.D.1, Sun Ja Choi, Ph.D.2,3,
Chang Deok Kim, Ph.D.1, Tae-Jin Yoon, M.D., Jeung-Hoon Lee, M.D.1
Department of Dermatology and Institute of Health Sciences, School of Medicine, Gyeongsang National University, Jinju, 1Department of
Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, 2Department of Visual
Design, Daeduk University, Daejeon, 3Department of Advanced Organic Materials Engineering, Chonbuk National University, Jeonju,
Background: Through differentiation processes, keratino-
cytes provide a physical barrier to our bodies and control skin
features such as moisturization, wrinkles and pigmentation.
Keratinocyte differentiation is disturbed in several skin
diseases such as psoriasis and atopic dermatitis. Objective:
The aim of this study is to evaluate the keratinocyte
differentiation-enhancing effect of rose absolute oil (RAO).
Methods: Primary cultured human normal keratinocytes
were treated with RAO, and differentiation then checked by
the expression of marker genes. Results: RAO did not induce
cytotoxicity on cultured keratinocytes at a dose of 10μM.
The level of involucrin, an early marker for keratinocyte
differentiation, was significantly increased by RAO. Con-
comitantly, RAO increased involucrin promoter activity,
indicating that RAO increased involucrin gene expression at
the mRNA level. Furthermore, RAO increased the level of
filaggrin in cultured keratinocytes, and in the granular layer
of mouse skin. In line with these results, RAO decreased the
proliferation of keratinocytes cultured in vitro. When RAO
was applied topically on the tape-stripped mouse skins, it
accelerated the recovery of disturbed barrier function.
Conclusion: These results suggest that RAO may be
applicable for the control of skin texture and keratinocyte
differentiation-related skin diseases. (Ann Dermatol 22(3)
Differentiation, Filaggrin, Keratinocyte, Rose absolute oil
In the epidermis, keratinocytes bear most responsibility for
maintaining structure and homeostasis. Epidermal kera-
tinocytes provide the rigid stratified structure through a
sophisticated differentiation program1,2. Keratinocyte di-
fferentiation involves the process of cell cycle arrest and
the onset of expression of numerous genes, resulting in,
characteristically, 4 layers of epidermis (stratum basale,
stratum spinosum, stratum granulosum and stratum
corneum)3,4. The transition from basal cells to corneocytes
is a complex process that requires the simultaneous
activation and inactivation of a wide variety of genes5. It
has been established that many genes such as involucrin,
loricrin and filaggrin are expressed in a temporally regul-
ated manner during keratinocyte differentiation6.
Dysregulated keratinocyte differentiation is also closely
related with several skin diseases including psoriasis and
atopic dermatitis7-9. Interestingly, such complex inflamma-
tory skin diseases are associated with hyperproliferation of
keratinocytes and disruption of skin barrier function,
resulting in exacerbation of immunologic reaction and
inflammation. Concordantly, disruption of skin barrier
function leads to excessive dry skin, which may be
another exacerbating factor for differentiation-related skin
diseases10. To date, major modalities for these skin dis-
eases are linked to the inactivation of immune reactions,
Keratinocyte Differentiation by Rose Absolute Oil
Vol. 22, No. 3, 2010
27. Proksch E, Brandner JM, Jensen JM. The skin: an indi-
spensable barrier. Exp Dermatol 2008;17:1063-1072.
28. Ruether A, Stoll M, Schwarz T, Schreiber S, Fölster-Holst R.
Filaggrin loss-of-function variant contributes to atopic
dermatitis risk in the population of Northern Germany. Br J
29. Proksch E, Fölster-Holst R, Jensen JM. Skin barrier function,
epidermal proliferation and differentiation in eczema. J
Dermatol Sci 2006;43:159-169.
30. Ulusoy S, Boşgelmez-Tinaz G, Secilmis-Canbay H. Toco-
pherol, carotene, phenolic contents and antibacterial pro-
perties of rose essential oil, hydrosol and absolute. Curr
31. Edlundh-Rose E, Kupershmidt I, Gustafsson AC, Parasassi T,
Serafino A, Bracci-Laudiero L, et al. Gene expression
analysis of human epidermal keratinocytes after N-acetyl
L-cysteine treatment demonstrates cell cycle arrest and
increased differentiation. Pathobiology 2005;72:203-212.
32. Balasubramanian S, Sturniolo MT, Dubyak GR, Eckert RL.
Human epidermal keratinocytes undergo (−)-epigallocate-
chin-3-gallate-dependent differentiation but not apoptosis.