Dielectrophoretic separation of mouse melanoma clones

Biomicrofluidics (Impact Factor: 3.36). 06/2010; 4(2). DOI: 10.1063/1.3447702
Source: PubMed


Dielectrophoresis (DEP) is employed to differentiate clones of mouse melanoma B16F10 cells. Five clones were tested on microelectrodes. At a specific excitation frequency, clone 1 showed a different DEP response than the other four. Growth rate, melanin content, recovery from cryopreservation, and in vitro invasive studies were performed. Clone 1 is shown to have significantly different melanin content and recovery rate from cryopreservation. This paper reports the ability of DEP to differentiate between two malignant cells of the same origin. Different DEP responses of the two clones could be linked to their melanin content.

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Available from: Stephen Beebe, Feb 10, 2014
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    • "In most cases, several different experimental conditions are tested and the purity and recovery of the results are analyzed to determine the optimum conditions. Two notable exceptions are the work by Gascoyne et al., [28] and Sabuncu and Beskok [29]. They have developed a separability parameter to measure the expected difference in the pDEP and nDEP responses of the target cells. "
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