Activity Paradigm to Induce Drosophila Larval Neuromuscular Junction Growth

ArticleinCold Spring Harbor Protocols 2010(8):pdb.prot5473 · August 2010with2 Reads
Impact Factor: 4.63 · DOI: 10.1101/pdb.prot5473 · Source: PubMed

    Abstract

    This article describes a method for spaced stimulation of Drosophila motor neurons. The neurons can be stimulated for short periods of time (~2 h) and then fixed (or imaged live). The method allows one to examine the immediate effects of altering activity on synaptic protein localization as well as neuromuscular junction structure and function.