Activity Paradigm to Induce Drosophila Larval Neuromuscular Junction Growth
This article describes a method for spaced stimulation of Drosophila motor neurons. The neurons can be stimulated for short periods of time (~2 h) and then fixed (or imaged live). The method allows one to examine the immediate effects of altering activity on synaptic protein localization as well as neuromuscular junction structure and function.
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