Article

Alterations in the Host Defense Properties of Human Milk Following Prolonged Storage or Pasteurization

Division of Pulmonary Biology and Neonatology, Cincinnati Children's Hospital Medical Center,Cincinnati, OH, USA.
Journal of pediatric gastroenterology and nutrition (Impact Factor: 2.63). 09/2010; 51(3):347-52. DOI: 10.1097/MPG.0b013e3181e07f0a
Source: PubMed

ABSTRACT

Preterm infants are often fed pasteurized donor milk or mother's milk that has been stored frozen for up to 4 weeks. Our objectives were to assess the impact of pasteurization or prolonged storage at -20 degrees C on the immunologic components of human milk and the capability of the different forms of human milk to support bacterial proliferation.
The concentrations and activities of major host defense proteins in the whey fractions of mother's milk stored for 4 weeks at -20 degrees C or pasteurized human donor milk were compared with freshly expressed human milk. Proliferation of bacteria incubated in the 3 forms of human milk was assessed.
Relative to freshly expressed human milk, the concentrations of lysozyme, lactoferrin, lactoperoxidase, and secretory immunoglobulin A were reduced 50% to 82% in pasteurized donor milk and the activities of lysozyme and lactoperoxidase were 74% to 88% lower (P < 0.01). Proliferation of bacterial pathogens in pasteurized donor milk was enhanced 1.8- to 4.6-fold compared with fresh or frozen human milk (P < 0.01).
The immunomodulatory proteins in human milk are reduced by pasteurization and, to a lesser extent, by frozen storage, resulting in decreased antibacterial capability. Stringent procedure to minimize bacterial contamination is essential during handling of pasteurized milk.

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    • ") and enzymes (lysozyme, lactoperoxidase, lipoprotein lipase, bile salt stimulated lipase) (Akinbi et al., 2010; Henderson, Fay, & Hamosh, 1998). Besides these alterations, heat treatments together with freeze-thaw cycles of human milk can induce the disruption of the milk fat globules (Vieira et al., 2011; Wardell, Hill, & Dsouza, 1981), protein denaturation and some aggregation of proteins on the fat globule membrane (Raikos, 2010; Ye, Singh, Taylor, & Anema, 2004). "
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    • ") . Furthermore , Akinbi et al . ( 2010 ) docu - mented that effectiveness of the immune proper - ties of human milk is reduced with freezing and pasteurization , and the risk of bacterial prolifera - tion is increased ( Akinbi et al . ) ."
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